Song K B, Joo H K, Rhee S K
Metabolic Engineering Laboratory, Genetic Engineering Research Institute, KIST, Taejon, South Korea.
Biochim Biophys Acta. 1993 Jun 25;1173(3):320-4. doi: 10.1016/0167-4781(93)90130-6.
The extracellular levansucrase gene (levU) was cloned from the genomic DNA of Zymomonas mobilis ZM1 and the nucleotide sequence of the levU structural gene was determined. The 3.1 kb EcoRV-polylinker fusion DNA fragment containing the levU gene had an open reading frame of 1272 bps and the deduced amino acid sequence was 423 residues long with a molecular weight of 46,725. Although this protein exhibited little similarity with other known levansucrases, several well-conserved regions were observed (1-5 regions).
从运动发酵单胞菌ZM1的基因组DNA中克隆出细胞外蔗糖蔗糖酶基因（levU），并测定了levU结构基因的核苷酸序列。含有levU基因的3.1 kb EcoRV-多克隆位点融合DNA片段具有1272个碱基对的开放阅读框，推导的氨基酸序列长度为423个残基，分子量为46,725。尽管该蛋白与其他已知的蔗糖蔗糖酶几乎没有相似性，但观察到了几个保守区域（1-5区）。
