Tanaka N, Ikeda T, Oka A
Bioassay Laboratory, Daicel Chemical Industries, Ltd, Hyogo, Japan.
Biosci Biotechnol Biochem. 1994 Mar;58(3):548-51. doi: 10.1271/bbb.58.548.
The rol genes of root-inducing plasmids (pRi) are essential for tumorigenesis on various dicotyledonous plants. The nucleotide sequences of the rol genes together with their flanking regions from the mikimopine-type pRi1724 were analyzed and compared with the corresponding portions of the agropine-type pRiA4 and the mannopine-type pRi8196 that were previously reported [Slightom et al., J. Biol. Chem., 261, 108-121 (1986); Hansen et al., Proc. Natl. Acad. Sci. U.S.A., 88, 7763-7767 (1991)]. The sequenced region of 8967 bp contained seven ORFs, five on the upper strand and two on the lower one. These ORFs resembled the ORFs 8, 10 (rolA), 11 (rolB), 12 (rolC), 13, 13a, and 14 of pRiA4 and pRi8196. However, the similarity of the rolABC genes, particularly rolA, among the three plasmids, was considerably lower than that of other ORFs. The spacer regions between the ORFs were also conserved, but to a smaller extent than ORFs, among the three plasmids.
发根质粒（pRi）的rol基因对于多种双子叶植物的肿瘤发生至关重要。分析了米基莫平型pRi1724的rol基因及其侧翼区域的核苷酸序列，并与先前报道的农杆碱型pRiA4和甘露碱型pRi8196的相应部分进行了比较[Slightom等人，《生物化学杂志》，261，108 - 121（1986）；Hansen等人，《美国国家科学院院刊》，88，7763 - 7767（1991）]。8967 bp的测序区域包含7个开放阅读框（ORF），其中5个在上链，2个在下链。这些开放阅读框类似于pRiA4和pRi8196的开放阅读框8、10（rolA）、11（rolB）、12（rolC）、13、13a和14。然而，这三种质粒中rolABC基因，特别是rolA基因的相似性，远低于其他开放阅读框。开放阅读框之间的间隔区域在这三种质粒中也保守，但程度小于开放阅读框。
