Kunik T, Salomon R, Zamir D, Navot N, Zeidan M, Michelson I, Gafni Y, Czosnek H
Department of Genetics, Agricultural Research Organization, Bet Dagan, Israel.
Biotechnology (N Y). 1994 May;12(5):500-4. doi: 10.1038/nbt0594-500.
The tomato yellow leaf curl virus (TYLCV) gene that encodes the capsid protein (V1) was placed under transcriptional control of the cauliflower mosaic virus 35S promoter and cloned into an Agrobacterium Ti-derived plasmid and used to transform plants from an interspecific tomato hybrid, Lycopersicon esculentum X L. pennellii (F1), sensitive to the TYLCV disease. When transgenic F1 plants, expressing the V1 gene, were inoculated with TYLCV using whiteflies fed on TYLCV-infected plants, they responded either as untransformed tomato or showed expression of delayed disease symptoms and recovery from the disease with increasingly more resistance upon repeated inoculation. Transformed plants that were as sensitive to inoculation as untransformed controls expressed the V1 gene at the RNA level only. All the transformed plants that recovered from disease expressed the TYLCV capsid protein.
编码衣壳蛋白（V1）的番茄黄化曲叶病毒（TYLCV）基因被置于花椰菜花叶病毒35S启动子的转录控制之下，并克隆到根癌农杆菌Ti衍生质粒中，用于转化对TYLCV病敏感的种间番茄杂种（Lycopersicon esculentum X L. pennellii，F1）的植株。当用取食TYLCV感染植株的粉虱对表达V1基因的转基因F1植株接种TYLCV时，它们的反应要么如同未转化的番茄，要么表现出延迟的病害症状，并随着反复接种而对病害的抵抗力增强，最终从病害中恢复。对接种同样敏感的转化植株仅在RNA水平上表达V1基因。所有从病害中恢复的转化植株均表达了TYLCV衣壳蛋白。
