Stephanopoulos G
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
Curr Opin Biotechnol. 1994 Feb;5(2):196-200. doi: 10.1016/s0958-1669(05)80036-9.
Metabolic engineering is not another form of classic manipulation of intermediary metabolism; rather, it is the purposeful design of metabolic networks. The metabolic engineering approach examines biochemical reactions in their entirety, rather than individually, and is concerned with the construction of novel pathways, the thermodynamic feasibility of pathways, and the location of limiting branch-point(s) and enzymatic reaction(s) in a reaction network. The ultimate goal is the construction of strains with superior yield and productivity obtained through a convergent evolutionary process. Progress has been made toward this objective within the framework of Metabolic Control Analysis through the development of methodologies for the experimental determination of elasticities and flux control coefficients.
代谢工程并非经典的中间代谢操纵的另一种形式；相反，它是代谢网络的有目的设计。代谢工程方法是从整体而非单个角度研究生化反应，关注新途径的构建、途径的热力学可行性以及反应网络中限制分支点和酶促反应的位置。最终目标是构建通过趋同进化过程获得的具有优异产量和生产力的菌株。在代谢控制分析的框架内，通过开发用于实验测定弹性和通量控制系数的方法，已朝着这一目标取得了进展。
