State Key Laboratory of Food Science and Technology, Jiangnan University , Wuxi 214122, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University , Wuxi 214122, China.
Chem Rev. 2018 Jan 10;118(1):4-72. doi: 10.1021/acs.chemrev.6b00804. Epub 2017 Apr 26.
Chemical synthesis is a well established route for producing many chemicals on a large scale, but some drawbacks still exist in this process, such as unstable intermediates, multistep reactions, complex process control, etc. Biobased production provides an attractive alternative to these challenges, but how to make cells into efficient factories is challenging. As a key enabling technology to develop efficient cell factories, design-construction-evaluation-optimization (DCEO) biotechnology, which incorporates the concepts and techniques of pathway design, pathway construction, pathway evaluation, and pathway optimization at the systems level, offers a conceptual and technological framework to exploit potential pathways, modify existing pathways and create new pathways for the optimal production of desired chemicals. Here, we summarize recent progress of DCEO biotechnology and examples of its application, and provide insights as to when, what and how different strategies should be taken. In addition, we highlight future perspectives of DCEO biotechnology for the successful establishment of biorefineries.
化学合成是大规模生产许多化学品的成熟途径，但该过程仍存在一些缺点，例如不稳定的中间体、多步反应、复杂的过程控制等。生物基生产为应对这些挑战提供了一个有吸引力的替代方案，但如何使细胞成为高效的工厂是具有挑战性的。作为开发高效细胞工厂的关键使能技术，设计-构建-评估-优化（DCEO）生物技术将途径设计、途径构建、途径评估和途径优化的概念和技术整合到系统层面，为利用潜在途径、修改现有途径和创建新途径以优化所需化学品的生产提供了概念和技术框架。在这里，我们总结了 DCEO 生物技术的最新进展和应用实例，并就何时、采用何种策略以及如何采用不同策略提供了见解。此外，我们还强调了 DCEO 生物技术在成功建立生物精炼厂方面的未来展望。
