Brown J C, Ward L J, Davey G P
New Zealand Dairy Research Institute, Palmerston North.
Lett Appl Microbiol. 1994 May;18(5):292-3. doi: 10.1111/j.1472-765x.1994.tb00872.x.
Intact bacteriophage of Lactococcus lactis were recovered from small volumes of lysate by centrifugation at 15,000 g without precipitation with polyethylene glycol and sodium chloride, or ultracentrifugation in a caesium chloride gradient. DNA was then extracted and purified by standard protocols. This DNA was readily digested with restriction endonucleases and used successfully in hybridization experiments.
通过在15,000 g下离心，无需用聚乙二醇和氯化钠沉淀，或在氯化铯梯度中进行超速离心，即可从小体积裂解物中回收完整的乳酸乳球菌噬菌体。然后通过标准方案提取和纯化DNA。该DNA很容易被限制性内切酶消化，并成功用于杂交实验。
