Ishida M, Kawashima H, Sato K, Hashiguchi K, Ito H, Enei H, Nakamori S
Central Research Laboratories of Ajinomoto Co., Ltd., Kanagawa, Japan.
Biosci Biotechnol Biochem. 1994 Apr;58(4):768-70. doi: 10.1271/bbb.58.768.
When a Brevibacterium lactofermentum L-threonine producer that accumulated L-lysine as well, was transformed with a recombinant plasmid carrying the indigenous hom, thrB, and thrC genes, delayed growth and plasmid instability were observed. Addition of organic nutrients, vitamins (thiamine.HCl and d-biotin), and NaCl under the optimal culture conditions solved these problems and further increased threonine production in a small jar fermentor.
当一株同时积累L-赖氨酸的乳糖发酵短杆菌L-苏氨酸生产菌用携带其自身的hom、thrB和thrC基因的重组质粒进行转化时，观察到生长延迟和质粒不稳定。在最佳培养条件下添加有机营养物、维生素（盐酸硫胺素和d-生物素）和氯化钠解决了这些问题，并在小型罐式发酵罐中进一步提高了苏氨酸产量。
