Yamano S, Ishii T, Nakagawa M, Ikenaga H, Misawa N
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama-shi, Japan.
Biosci Biotechnol Biochem. 1994 Jun;58(6):1112-4. doi: 10.1271/bbb.58.1112.
We have engineered a conventional yeast, Saccharomyces cerevisiae, to confer a novel biosynthetic pathway for the production of beta-carotene and lycopene by introducing the bacterial carotenoid biosynthesis genes, which are individually surrounded by the promoters and terminators derived from S. cerevisiae. beta-Carotene and lycopene accumulated in the cells of this yeast, which was considered to be a result of the carbon flow for the ergosterol biosynthetic pathway being partially directed to the pathway for the carotenoid production.
我们对传统酵母酿酒酵母进行了改造，通过引入细菌类胡萝卜素生物合成基因赋予其一条用于生产β-胡萝卜素和番茄红素的新生物合成途径，这些基因分别被源自酿酒酵母的启动子和终止子所包围。β-胡萝卜素和番茄红素在这种酵母的细胞中积累，这被认为是麦角固醇生物合成途径的碳流部分转向类胡萝卜素生产途径的结果。
