Miura Y, Kondo K, Saito T, Shimada H, Fraser P D, Misawa N
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Kanagawa, Japan.
Appl Environ Microbiol. 1998 Apr;64(4):1226-9. doi: 10.1128/AEM.64.4.1226-1229.1998.
The food-grade yeast Candida utilis has been engineered to confer a novel biosynthetic pathway for the production of carotenoids such as lycopene, beta-carotene, and astaxanthin. The exogenous carotenoid biosynthesis genes were derived from the epiphytic bacterium Erwinia uredovora and the marine bacterium Agrobacterium aurantiacum. The carotenoid biosynthesis genes were individually modified based on the codon usage of the C. utilis glyceraldehyde 3-phosphate dehydrogenase gene and expressed in C. utilis under the control of the constitutive promotes and terminators derived from C. utilis. The resultant yeast strains accumulated lycopene, beta-carotene, and astaxanthin in the cells at 1.1, 0.4, and 0.4 mg per g (dry weight) of cells, respectively. This was considered to be a result of the carbon flow into ergosterol biosynthesis being partially redirected to the nonendogenous pathway for carotenoid production.
食品级酵母产朊假丝酵母已被改造，以赋予其一条用于生产类胡萝卜素（如番茄红素、β-胡萝卜素和虾青素）的新型生物合成途径。外源类胡萝卜素生物合成基因来源于附生细菌草生欧文氏菌和海洋细菌橙色农杆菌。基于产朊假丝酵母甘油醛-3-磷酸脱氢酶基因的密码子使用情况，对类胡萝卜素生物合成基因进行了单独修饰，并在源自产朊假丝酵母的组成型启动子和终止子的控制下在产朊假丝酵母中表达。所得酵母菌株在细胞中分别以每克（干重）细胞1.1毫克、0.4毫克和0.4毫克的量积累番茄红素、β-胡萝卜素和虾青素。这被认为是流入麦角固醇生物合成的碳流部分重定向到类胡萝卜素生产的非内源途径的结果。
