食品酵母产朊假丝酵母中类胡萝卜素番茄红素、β-胡萝卜素和虾青素的生产。
Production of the carotenoids lycopene, beta-carotene, and astaxanthin in the food yeast Candida utilis.
作者信息
Miura Y, Kondo K, Saito T, Shimada H, Fraser P D, Misawa N
机构信息
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Kanagawa, Japan.
出版信息
Appl Environ Microbiol. 1998 Apr;64(4):1226-9. doi: 10.1128/AEM.64.4.1226-1229.1998.
Abstract
The food-grade yeast Candida utilis has been engineered to confer a novel biosynthetic pathway for the production of carotenoids such as lycopene, beta-carotene, and astaxanthin. The exogenous carotenoid biosynthesis genes were derived from the epiphytic bacterium Erwinia uredovora and the marine bacterium Agrobacterium aurantiacum. The carotenoid biosynthesis genes were individually modified based on the codon usage of the C. utilis glyceraldehyde 3-phosphate dehydrogenase gene and expressed in C. utilis under the control of the constitutive promotes and terminators derived from C. utilis. The resultant yeast strains accumulated lycopene, beta-carotene, and astaxanthin in the cells at 1.1, 0.4, and 0.4 mg per g (dry weight) of cells, respectively. This was considered to be a result of the carbon flow into ergosterol biosynthesis being partially redirected to the nonendogenous pathway for carotenoid production.
摘要
食品级酵母产朊假丝酵母已被改造，以赋予其一条用于生产类胡萝卜素（如番茄红素、β-胡萝卜素和虾青素）的新型生物合成途径。外源类胡萝卜素生物合成基因来源于附生细菌草生欧文氏菌和海洋细菌橙色农杆菌。基于产朊假丝酵母甘油醛-3-磷酸脱氢酶基因的密码子使用情况，对类胡萝卜素生物合成基因进行了单独修饰，并在源自产朊假丝酵母的组成型启动子和终止子的控制下在产朊假丝酵母中表达。所得酵母菌株在细胞中分别以每克（干重）细胞1.1毫克、0.4毫克和0.4毫克的量积累番茄红素、β-胡萝卜素和虾青素。这被认为是流入麦角固醇生物合成的碳流部分重定向到类胡萝卜素生产的非内源途径的结果。
相似文献
1
Production of the carotenoids lycopene, beta-carotene, and astaxanthin in the food yeast Candida utilis.食品酵母产朊假丝酵母中类胡萝卜素番茄红素、β-胡萝卜素和虾青素的生产。
Appl Environ Microbiol. 1998 Apr;64(4):1226-9. doi: 10.1128/AEM.64.4.1226-1229.1998.
2
Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts.用于在非类胡萝卜素生成细菌和酵母中生产类胡萝卜素的代谢工程。
J Biotechnol. 1997 Jan 3;59(3):169-81. doi: 10.1016/s0168-1656(97)00154-5.
3
Production of lycopene by the food yeast, Candida utilis that does not naturally synthesize carotenoid.由不能天然合成类胡萝卜素的食用酵母产朊假丝酵母生产番茄红素。
Biotechnol Bioeng. 1998;58(2-3):306-8.
4
Metabolic engineering for production of beta-carotene and lycopene in Saccharomyces cerevisiae.用于在酿酒酵母中生产β-胡萝卜素和番茄红素的代谢工程。
Biosci Biotechnol Biochem. 1994 Jun;58(6):1112-4. doi: 10.1271/bbb.58.1112.
5
Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level.海洋细菌类胡萝卜素生物合成基因簇的结构与功能分析以及在基因水平上提出的虾青素生物合成途径
J Bacteriol. 1995 Nov;177(22):6575-84. doi: 10.1128/jb.177.22.6575-6584.1995.
6
Production of beta-carotene in Zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis genes from Erwinia uredovora.通过导入来自丁香假单胞菌的生物合成基因，在运动发酵单胞菌和根癌农杆菌中生产β-胡萝卜素。
Appl Environ Microbiol. 1991 Jun;57(6):1847-9. doi: 10.1128/aem.57.6.1847-1849.1991.
7
Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway.通过类异戊二烯途径的代谢工程提高食用酵母产朊假丝酵母的类胡萝卜素产量。
Appl Environ Microbiol. 1998 Jul;64(7):2676-80. doi: 10.1128/AEM.64.7.2676-2680.1998.
8
Construction of new Pichia pastoris X-33 strains for production of lycopene and β-carotene.构建新型毕赤酵母 X-33 菌株用于生产番茄红素和β-胡萝卜素。
Appl Microbiol Biotechnol. 2012 Mar;93(6):2483-92. doi: 10.1007/s00253-011-3764-7. Epub 2011 Dec 9.
9
Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli.通过对在大肠杆菌中表达的基因产物进行功能分析来阐明胡萝卜软腐欧文氏菌类胡萝卜素生物合成途径。
J Bacteriol. 1990 Dec;172(12):6704-12. doi: 10.1128/jb.172.12.6704-6712.1990.
10
In vitro characterization of astaxanthin biosynthetic enzymes.虾青素生物合成酶的体外特性研究
J Biol Chem. 1997 Mar 7;272(10):6128-35. doi: 10.1074/jbc.272.10.6128.
引用本文的文献
1
Engineered Strains for Enhanced Astaxanthin Production.用于提高虾青素产量的工程菌株。
Life (Basel). 2025 May 20;15(5):813. doi: 10.3390/life15050813.
2
Last Decade Insights in Exploiting Marine Microorganisms as Sources of New Bioactive Natural Products.过去十年间利用海洋微生物作为新型生物活性天然产物来源的研究洞察
Mar Drugs. 2025 Mar 7;23(3):116. doi: 10.3390/md23030116.
3
Arts, cultural heritage, sciences, and micro-/bio-/technology: Impact of biomaterials and biocolorants from antiquity till today!艺术、文化遗产、科学与微/生物/技术：从古代至今生物材料和生物色素的影响！
J Ind Microbiol Biotechnol. 2024 Jan 9;51. doi: 10.1093/jimb/kuae049.
4
Biotechnological advances for improving natural pigment production: a state-of-the-art review.用于提高天然色素产量的生物技术进展：最新综述
Bioresour Bioprocess. 2022 Jan 28;9(1):8. doi: 10.1186/s40643-022-00497-4.
5
Phytochemical Content of Malus floribunda: In Vitro and Molecular Docking Studies.绵苹果的植物化学成分：体外和分子对接研究。
Appl Biochem Biotechnol. 2024 Aug;196(8):5198-5218. doi: 10.1007/s12010-023-04826-x. Epub 2023 Dec 28.
6
Natural Pigments Production and Their Application in Food, Health and Other Industries.天然色素的生产及其在食品、健康和其他行业中的应用。
Nutrients. 2023 Apr 16;15(8):1923. doi: 10.3390/nu15081923.
7
Evaluation of Various Strains for Enhanced Lycopene Production.评价不同菌株提高番茄红素产量的能力。
J Microbiol Biotechnol. 2023 Jul 28;33(7):973-979. doi: 10.4014/jmb.2302.02003. Epub 2023 Apr 17.
8
CRISPRi-mediated tunable control of gene expression level with engineered single-guide RNA in Escherichia coli.CRISPRi 介导的工程化单指导 RNA 调控大肠杆菌中基因表达水平
Nucleic Acids Res. 2023 May 22;51(9):4650-4659. doi: 10.1093/nar/gkad234.
9
Carotenoids and Their Biosynthesis in Fungi.类胡萝卜素及其在真菌中的生物合成。
Molecules. 2022 Feb 21;27(4):1431. doi: 10.3390/molecules27041431.
10
Bacterial and fungal communities of traditional fermented Chinese soybean paste (Doujiang) and their properties.传统发酵中国豆瓣酱（豆酱）的细菌和真菌群落及其特性。
Food Sci Nutr. 2021 Aug 31;9(10):5457-5466. doi: 10.1002/fsn3.2505. eCollection 2021 Oct.
本文引用的文献
1
Production of lycopene by the food yeast, Candida utilis that does not naturally synthesize carotenoid.由不能天然合成类胡萝卜素的食用酵母产朊假丝酵母生产番茄红素。
Biotechnol Bioeng. 1998;58(2-3):306-8.
2
High-level expression of a sweet protein, monellin, in the food yeast Candida utilis.甜味蛋白莫内林在产朊假丝酵母中的高水平表达。
Nat Biotechnol. 1997 May;15(5):453-7. doi: 10.1038/nbt0597-453.
3
High copy number integration into the ribosomal DNA of the yeast Phaffia rhodozyma.高拷贝数整合到红酵母（Phaffia rhodozyma）的核糖体DNA中。
Gene. 1997 Jan 3;184(1):89-97. doi: 10.1016/s0378-1119(96)00579-3.
4
A transformation system for the yeast Candida utilis: use of a modified endogenous ribosomal protein gene as a drug-resistant marker and ribosomal DNA as an integration target for vector DNA.产朊假丝酵母的转化系统：利用修饰的内源性核糖体蛋白基因作为抗药标记以及核糖体DNA作为载体DNA的整合靶点
J Bacteriol. 1995 Dec;177(24):7171-7. doi: 10.1128/jb.177.24.7171-7177.1995.
5
Metabolic engineering for production of beta-carotene and lycopene in Saccharomyces cerevisiae.用于在酿酒酵母中生产β-胡萝卜素和番茄红素的代谢工程。
Biosci Biotechnol Biochem. 1994 Jun;58(6):1112-4. doi: 10.1271/bbb.58.1112.
6
Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level.海洋细菌类胡萝卜素生物合成基因簇的结构与功能分析以及在基因水平上提出的虾青素生物合成途径
J Bacteriol. 1995 Nov;177(22):6575-84. doi: 10.1128/jb.177.22.6575-6584.1995.
7
Intake of carotenoids and retinol in relation to risk of prostate cancer.类胡萝卜素和视黄醇的摄入量与前列腺癌风险的关系。
J Natl Cancer Inst. 1995 Dec 6;87(23):1767-76. doi: 10.1093/jnci/87.23.1767.
8
Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli.通过对在大肠杆菌中表达的基因产物进行功能分析来阐明胡萝卜软腐欧文氏菌类胡萝卜素生物合成途径。
J Bacteriol. 1990 Dec;172(12):6704-12. doi: 10.1128/jb.172.12.6704-6712.1990.
9
Studies of immunomodulating actions of carotenoids. I. Effects of beta-carotene and astaxanthin on murine lymphocyte functions and cell surface marker expression in in vitro culture system.类胡萝卜素免疫调节作用的研究。I. β-胡萝卜素和虾青素对体外培养系统中小鼠淋巴细胞功能及细胞表面标志物表达的影响
Nutr Cancer. 1991;16(2):93-105. doi: 10.1080/01635589109514148.
10
Production of food and fodder yeasts.食品和饲料酵母的生产。
Crit Rev Biotechnol. 1992;12(1-2):65-86. doi: 10.3109/07388559209069188.
Zotero 插件