一种荧光标记寡核苷酸的新合成方法及其在DNA测序中的应用。
A new method of synthesis of fluorescently labelled oligonucleotides and their application in DNA sequencing.
作者信息
Markiewicz W T, Gröger G, Rösch R, Zebrowska A, Markiewicz M, Klotz M, Hinz M, Godzina P, Seliger H
机构信息
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12, PL-61704 Poznan, Poland.
出版信息
Nucleic Acids Res. 1997 Sep 15;25(18):3672-80. doi: 10.1093/nar/25.18.3672.
Abstract
A new approach to the chemical synthesis of oligodeoxynucleotides bearing reporter functional groups at base residues of 3'-end nucleosides is reported. Applications of the 3'-end fluorescently labelled primers for automated DNA sequencing are shown.
摘要
报道了一种在3'-末端核苷的碱基残基上带有报告功能基团的寡聚脱氧核苷酸化学合成的新方法。展示了3'-末端荧光标记引物在自动化DNA测序中的应用。
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