Ballal S K, Isham K, Foote R S
Biology Department, Tennessee Technological University, Cookeville 38505.
SAAS Bull Biochem Biotechnol. 1994;7:40-3.
There is an increasing interest in using selected antisense oligodeoxynucleotides to control the ribosomal translational process in cells. The oligonucleotide hybridizes to a target RNA to inhibit its translation. In order to prevent the degradation of oligonucleotides by residual and constitutive ribonucleases, various molecular alterations to antisense oligos have been attempted. In this report, evidence is offered that a phosphorothioate-modified 16-mer synthetic oligodeoxynucleotide inhibits the synthesis of O6-methylguanine-DNA methyltransferase (MGMT) in cultured HeLa S3 cells within six hours after introduction.
人们对使用特定的反义寡脱氧核苷酸来控制细胞中的核糖体翻译过程越来越感兴趣。该寡核苷酸与靶RNA杂交以抑制其翻译。为了防止寡核苷酸被残留的和组成型核糖核酸酶降解，人们尝试了对反义寡核苷酸进行各种分子改造。在本报告中，有证据表明，一种硫代磷酸酯修饰的16聚体合成寡脱氧核苷酸在导入培养的HeLa S3细胞后6小时内抑制了O6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）的合成。
