Moyes A, Chronas G, Young H
Scottish Gonococcal Reference Laboratory, Department of Medical Microbiology, Edinburgh University Medical School, UK.
Lett Appl Microbiol. 1994 Oct;19(4):232-6. doi: 10.1111/j.1472-765x.1994.tb00951.x.
A rapid enzymatic method using chromogenic substrates for the rapid identification of pathogenic neisseria (Identicult-Neisseria, Scott Laboratories Inc., CA, USA) was tested in parallel with the rapid carbohydrate utilization test (RCUT) and the Phadebact Monoclonal GC Test against 198 consecutive clinical isolates of oxidase-positive Gram-negative diplococci (118 Neisseria gonorrhoeae, 76 N. meningitidis and four N. lactamica). On initial testing the Identicult-Neisseria gave a 95% overall concordance (97.5% N. gonorrhoeae, 90.8% N. meningitidis) with the RCUT and Phadebact tests; the corresponding figures after repeat testing were 98% overall concordance (98.3% N. gonorrhoeae, 97.4% N. meningitidis). Two of the three strains of N. gonorrhoeae mis-identified as N. meningitidis on primary testing were also mis-identified on repeat testing. Seven strains of N. meningitidis were mis-identified on initial testing (six as Moraxella catarrhalis and one as N. lactamica) and two on repeat testing (both as Mor. catarrhalis). We conclude that the Identicult-Neisseria is not sufficiently reliable for the culture confirmation of gonococci and meningococci.
使用显色底物的快速酶法用于快速鉴定致病性奈瑟菌（Identicult - Neisseria，美国加利福尼亚州斯科特实验室公司），与快速碳水化合物利用试验（RCUT）和Phadebact单克隆GC试验同时针对198株连续的氧化酶阳性革兰氏阴性双球菌临床分离株（118株淋病奈瑟菌、76株脑膜炎奈瑟菌和4株乳糖奈瑟菌）进行了测试。在初次测试时，Identicult - Neisseria与RCUT和Phadebact试验的总体一致性为95%（淋病奈瑟菌为97.5%，脑膜炎奈瑟菌为90.8%）；重复测试后的相应数字为总体一致性98%（淋病奈瑟菌为98.3%，脑膜炎奈瑟菌为97.4%）。初次测试时被误鉴定为脑膜炎奈瑟菌的三株淋病奈瑟菌中的两株在重复测试时也被误鉴定。七株脑膜炎奈瑟菌在初次测试时被误鉴定（六株被误鉴定为卡他莫拉菌，一株被误鉴定为乳糖奈瑟菌），两株在重复测试时被误鉴定（均被误鉴定为卡他莫拉菌）。我们得出结论，Identicult - Neisseria在淋病奈瑟菌和脑膜炎奈瑟菌的培养确认方面不够可靠。
