Moon J H, Watanabe N, Sakata K, Yagi A, Ina K, Luo S
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Biosci Biotechnol Biochem. 1994 Sep;58(9):1742-4. doi: 10.1271/bbb.58.1742.
New glycosidic aroma precursors (1 and 2) of the main volatile constituents, trans- and cis-linalool 3,6-oxides (linalool oxides I and II), were isolated from oolong tea leaves (Camellia sinensis var. sinensis cv. Maoxie). The isolation was guided by an enzymatic hydrolysis with acetone powder prepared from fresh tea leaves (cv. Yabukita) followed by GC or GC-MS analyses. Chromatographic purification of hot water extracts of the tea leaves on active charcoal, Amberlite XAD-2, and Sephadex LH-20 columns as well as HPLC gave two new glycosides, trans- and cis-linalool 3,6-oxide 6-O-beta-D-xylopyranosyl-beta-D-glucopyranosides (1 and 2).
从乌龙茶（茶树变种中华变种毛蟹）叶片中分离出了主要挥发性成分反式和顺式芳樟醇3,6 -氧化物（芳樟醇氧化物I和II）的新糖苷类香气前体（1和2）。分离过程以鲜茶叶（薮北品种）制备的丙酮粉进行酶解，随后进行气相色谱或气相色谱 - 质谱分析为指导。通过在活性炭、Amberlite XAD - 2和Sephadex LH - 20柱上对茶叶热水提取物进行色谱纯化以及高效液相色谱法，得到了两种新的糖苷，反式和顺式芳樟醇3,6 -氧化物6 - O - β - D - 吡喃木糖基 - β - D - 吡喃葡萄糖苷（1和2）。
