采用聚合酶链反应基因扩增技术检测粪便标本中产生Vero细胞毒素的大肠杆菌和1型痢疾志贺菌。
The detection of Vero cytotoxin-producing Escherichia coli and Shigella dysenteriae type 1 in faecal specimens using polymerase chain reaction gene amplification.
作者信息
Thomas A, Jiggle B, Smith H R, Rowe B
机构信息
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
出版信息
Lett Appl Microbiol. 1994 Dec;19(6):406-9. doi: 10.1111/j.1472-765x.1994.tb00968.x.
Fifty consecutive faecal specimens received by the LEP were examined for the presence of Vero cytotoxin (VT) genes by polymerase chain reaction (PCR) gene amplification. Nineteen were positive by PCR and from 16 of these, VT positive Escherichia coli O157 were isolated. The remaining three samples were positive for VT genes by PCR but VTEC were not isolated. In a preliminary experiment, Shigella dysenteriae type 1 was isolated from a case of bloody diarrhoea following a positive amplification result.
伦敦东部病原体参考实验室（LEP）收到的50份连续粪便标本，通过聚合酶链反应（PCR）基因扩增检测是否存在志贺毒素（VT）基因。其中19份PCR检测呈阳性，从这19份标本中的16份分离出VT阳性的大肠杆菌O157。其余3份标本PCR检测VT基因呈阳性，但未分离出产志贺毒素大肠杆菌（VTEC）。在一项初步实验中，从一名血性腹泻患者身上分离出1型痢疾志贺菌，其扩增结果呈阳性。
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