用于鉴定大肠杆菌毒力基因的多重聚合酶链反应
Multiplex PCRs for identification of Escherichia coli virulence genes.
作者信息
Pass M A, Odedra R, Batt R M
机构信息
Department of Physiology and Pharmacology, The University of Queensland, St. Lucia, Queensland, 4072, Australia.
出版信息
J Clin Microbiol. 2000 May;38(5):2001-4. doi: 10.1128/JCM.38.5.2001-2004.2000.
Abstract
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.
摘要
开发了聚合酶链反应（PCR）来检测11种大肠杆菌毒力基因。引物能扩增各自的基因，且不会与其他基因发生交叉反应。在多重反应中保持了特异性；最少进行四次多重反应就能出色地扩增目标基因。这些反应成功鉴定了来自四只狗粪便中的大肠杆菌基因。
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