Nakahara K, Kitamura Y, Yamagishi Y, Shoun H, Yasui T
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Biosci Biotechnol Biochem. 1994 Dec;58(12):2193-6. doi: 10.1271/bbb.58.2193.
A levoglucosan (1,6-anhydro-beta-D-glucopyranose)-using bacterium, isolated from soil, was identified. It was shown to belong to the genus Arthrobacter and tentatively named Arthrobacter sp. I-552. A novel enzyme catalyzed the dehydrogenation of levoglucosan to form 1,6-anhydro-beta-D-ribo-hexopyranos-3-ulose (3-keto levoglucosan), using NAD+ as an electron acceptor, i.e. NAD+: 1,6-anhydro-beta-D-glucopyranose oxidoreductase (trivial name: levoglucosan dehydrogenase). This enzyme was purified and characterized. A possible reaction scheme for the glucose formation was proposed. This pathway for levoglucosan use is distinct from those in yeast and fungi.
从土壤中分离出了一株利用左旋葡聚糖（1,6-脱水-β-D-吡喃葡萄糖）的细菌，并对其进行了鉴定。结果表明它属于节杆菌属，暂命名为节杆菌I-552。一种新型酶催化左旋葡聚糖脱氢形成1,6-脱水-β-D-核糖己吡喃糖-3-酮（3-酮基左旋葡聚糖），以NAD⁺作为电子受体，即NAD⁺：1,6-脱水-β-D-吡喃葡萄糖氧化还原酶（俗名：左旋葡聚糖脱氢酶）。对该酶进行了纯化和表征。提出了一个可能的葡萄糖形成反应方案。这种利用左旋葡聚糖的途径与酵母和真菌中的不同。
