通过竞争性酶联免疫吸附测定法测量罗望子木葡聚糖衍生的寡糖。
Measurement of oligosaccharides derived from tamarind xyloglucan by competitive ELISA assay.
作者信息
Sone Y, Sato K
机构信息
Department of Food and Nutrition, Faculty of Human Life Science, Osaka City University, Japan.
出版信息
Biosci Biotechnol Biochem. 1994 Dec;58(12):2295-6. doi: 10.1271/bbb.58.2295.
PMID:7765725
Abstract
Xyloglucan oligosaccharides, which were derived from tamarind xyloglucan by cellulase digestion, were measured by a competitive ELISA method using antibodies raised against a complex of BSA and xyloglucan nonasaccharide. By this method, tamarind xyloglucan oligosaccharides could be measured in the range of 0.1 to 40 nmol/well.
摘要
通过纤维素酶消化罗望子木葡聚糖得到的木葡聚糖寡糖，采用针对牛血清白蛋白（BSA）与木葡聚糖九糖复合物产生的抗体，通过竞争酶联免疫吸附测定（ELISA）法进行测定。通过这种方法，罗望子木葡聚糖寡糖的测定范围为0.1至40 nmol/孔。
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