Kioukia N, Nienow A W, Emery A N, al-Rubeai M
BBSRC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
J Biotechnol. 1995 Jan 31;38(3):243-51. doi: 10.1016/0168-1656(94)00128-y.
Insect cell growth can be significantly improved by close attention to the conditions used in the inoculum stages. Initial cell concentration, spent medium carry over and inoculum phase withdrawal significantly influenced the growth kinetics of Spodoptera frugiperda (Sf9) cells. The percentage of cells infected with wild and recombinant baculovirus AcNPV and (in the later case) the beta-galactosidase yield in fresh medium was appreciably affected by the stage of the growth curve that cells were in when infected and by the multiplicity of infection (MOI). However, the cell density at the time of infection and the medium condition showed little direct influence on infectivity. There may, however, be an indirect influence in that these factors determine the relative distribution of cells in the cell cycle. The infectivity is then in turn affected by the relative frequency of cells in the G1, S and G2/M phases. Insect cell specific oxygen uptake rates (1.3-3.4 x 10(-17) mol per cell per s) were essentially similar to or less than those measured for hybridoma cells. However, when Sf9 cells were infected with baculovirus, the specific oxygen uptake rate increased by up to 40%.
通过密切关注接种阶段所使用的条件，昆虫细胞的生长可得到显著改善。初始细胞浓度、用过的培养基残留量和接种阶段的撤换显著影响了草地贪夜蛾（Sf9）细胞的生长动力学。感染野生型和重组杆状病毒AcNPV的细胞百分比以及（在后一种情况下）新鲜培养基中的β-半乳糖苷酶产量，受到感染时细胞所处生长曲线阶段以及感染复数（MOI）的显著影响。然而，感染时的细胞密度和培养基条件对感染性几乎没有直接影响。不过，这些因素可能会产生间接影响，因为它们决定了细胞在细胞周期中的相对分布。而感染性又会反过来受到处于G1、S和G2/M期细胞的相对频率的影响。昆虫细胞的特定氧摄取率（1.3 - 3.4×10⁻¹⁷摩尔/细胞/秒）与杂交瘤细胞所测得的基本相似或更低。然而，当Sf9细胞感染杆状病毒时，特定氧摄取率最多可提高40%。
