Rosinski M, Reid S, Nielsen L K
Department of Chemical Engineering, The University of Queensland, St Lucia, Qld 4072, Australia.
Biotechnol Prog. 2000 Sep-Oct;16(5):782-5. doi: 10.1021/bp000083n.
Rates of cell size increase are an important measure of success during the baculovirus infection process. Batch and fed batch cultures sustain large fluctuations in osmolarity that can affect the measured cell volume if this parameter is not considered during the sizing protocol. Where osmolarity differences between the sizing diluent and the culture broth exist, biased measurements of size are obtained as a result of the cell osmometer response. Spodoptera frugiperda (Sf9) cells are highly sensitive to volume change when subjected to a change in osmolarity. Use of the modified protocol with culture supernatants for sample dilution prior to sizing removed the observed error during measurement.
细胞大小增加率是杆状病毒感染过程中衡量成功与否的一项重要指标。分批培养和补料分批培养会使渗透压产生大幅波动，如果在测量细胞大小的方案中未考虑这一参数，就可能影响所测得的细胞体积。当大小测量稀释剂与培养液之间存在渗透压差异时，由于细胞渗透计的反应，会导致大小测量出现偏差。草地贪夜蛾（Sf9）细胞在渗透压发生变化时对体积变化高度敏感。在测量前使用改良方案，用培养上清液进行样品稀释，消除了测量过程中观察到的误差。
