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Clarification of animal cell cultures on a large scale by continuous centrifugation.

作者信息

Kempken R, Preissmann A, Berthold W

机构信息

Thomae GmbH (Boehringer Ingelheim Germany), Department of Biotechnological Production, Riss, Germany.

出版信息

J Ind Microbiol. 1995 Jan;14(1):52-7. doi: 10.1007/BF01570067.

DOI:10.1007/BF01570067
PMID:7766067
Abstract

Animal cells from 80-L and 2000-L fed batch fermentations were removed by a prototype disc stack centrifuge in order to achieve a fast and reliable separation of solids from large quantities of cell culture fluids. The clarification capacity was excellent for animal cells but particles remained in the liquid phase and affected further downstream processing of the cell-free harvest fluid. No significant loss of product was observed. A number of parameters were monitored to optimize process conditions for use with animal cells.

摘要

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本文引用的文献

1
Assessment of a disc stack centrifuge for use in mammalian cell separation.用于哺乳动物细胞分离的碟片式离心机的评估
Biotechnol Bioeng. 1995 Apr 20;46(2):132-8. doi: 10.1002/bit.260460206.
2
Industrial scale harvest of proteins from mammalian cell culture by tangential flow filtration.切向流过滤法从哺乳动物细胞培养物中工业规模提取蛋白质。
Biotechnol Bioeng. 1991 Aug 5;38(4):413-22. doi: 10.1002/bit.260380411.
3
Crossflow microfiltration of animal cells.动物细胞错流微滤。
Biotechnol Bioeng. 1991 Jan 20;37(2):121-6. doi: 10.1002/bit.260370205.
4
Successful development of recombinant DNA-derived pharmaceuticals.重组DNA衍生药物的成功研发。
Arzneimittelforschung. 1990 Nov;40(11):1274-83.
5
Safety and economic aspects of continuous mammalian cell culture.哺乳动物细胞连续培养的安全性和经济方面。
J Biotechnol. 1992 Jan;22(1-2):51-68. doi: 10.1016/0168-1656(92)90132-s.