Fontes C M, Hall J, Hirst B H, Hazlewood G P, Gilbert H J
Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, UK.
Appl Microbiol Biotechnol. 1995 Apr;43(1):52-7. doi: 10.1007/BF00170622.
The sensitivity of a range of cellulases and xylanases to proteolytic inactivation was investigated. The xylanases, all the Clostridium thermocellum cellulases and cellulase E from Pseudomonas fluorescens subsp. cellulosa exhibited no decrease in catalytic activity during a 3-h incubation with proteinases of the small intestine. Under these conditions, the control Escherichia coli enzymes analysed had half-lives of 4.3-13.5 min. The addition of substrate significantly decreased the sensitivity of proteinase-labile enzymes to inactivation. The significance of these data in relation to the use of cellulases and xylanases for improving animal nutrition is discussed.
研究了一系列纤维素酶和木聚糖酶对蛋白水解失活的敏感性。木聚糖酶、所有热纤梭菌纤维素酶以及来自荧光假单胞菌纤维素亚种的纤维素酶E在与小肠蛋白酶孵育3小时的过程中，催化活性均未降低。在这些条件下，所分析的对照大肠杆菌酶的半衰期为4.3 - 13.5分钟。添加底物显著降低了对蛋白酶敏感的酶的失活敏感性。讨论了这些数据对于使用纤维素酶和木聚糖酶改善动物营养的意义。
