Pushalkar S, Rao K K, Menon K
Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, India.
Curr Microbiol. 1995 May;30(5):255-8. doi: 10.1007/BF00295497.
The production of beta-glucosidase by Aspergillus terreus was investigated in liquid shake cultures. Enzyme production was maximum on the 7th day of growth (2.18 U/ml) with the initial pH of the medium in the range of 4.0-5.5. Cellulose (Sigmacell Type 100) at 1.0% (wt/vol) gave maximum beta-glucosidase activity among the various soluble and insoluble carbon sources tested. Potassium nitrate was a suitable nitrogen source for enzyme production. Triton X-100 at 0.15% (vol/vol) increased the enzyme levels of A. terreus. The test fungal strain showed an ability to ferment glucose to ethanol.
在液体摇瓶培养中研究了土曲霉β-葡萄糖苷酶的产生。在生长的第7天酶产量最高（2.18 U/ml），培养基的初始pH值在4.0 - 5.5范围内。在所测试的各种可溶性和不溶性碳源中，1.0%（重量/体积）的纤维素（SigmaCell 100型）产生的β-葡萄糖苷酶活性最高。硝酸钾是适合酶产生的氮源。0.15%（体积/体积）的吐温X-100提高了土曲霉的酶水平。受试真菌菌株表现出将葡萄糖发酵为乙醇的能力。
