Potential applications of lifetime-based, phase-modulation fluorimetry in bioprocess and clinical monitoring.

The measurement of analyte concentration is a critical part of successful bioreactor and clinical monitoring. Although strategies exist for measuring the majority of relevant analytes, industrial on-line bioreactor control is carried out primarily by measurement and control of pH, pO2 and, in some cases, cell density. This is because the available technology cannot be easily and inexpensively adapted to (a) measure the analyte in an aseptic manner and/or allow for remote sensing, and (b) measure in real time so that on-line control is possible. Similar issues need to be addressed for biosensors for clinical applications. A rapidly emerging technology that has the potential of meeting these challenges is liftime-based phase-modulation fluorimetry, an optical technique that uses the measurement of fluorescence lifetime rather than intensity for determining the concentration of an analyte.