Dibirdik I, Durak N, Kişlaoğlu E, Kutluay T, Aytemiz C
Department of Biochemistry and Clinical Biochemistry, Gülhane Military Medical Academy and Medical School, Ankara, Turkey.
Burns. 1995 Mar;21(2):130-5. doi: 10.1016/0305-4179(95)92138-3.
In this study on patients with thermal trauma, we examined the effects of standard therapy plus prophylactic polyclonal immunoglobulin G (IgG) treatment on humoral and cellular contents, cell phenotype and function of the immune system, and compare these with those found in patients receiving only standard therapy. The quantitative, peripheral-blood mononuclear cell panel shows a decrease in the total number of T-lymphocytes and an increase in the natural killer (NK) cells of standard therapy patients 3 weeks following the burn. We found that intravenous IgG treatment does not have an important effect on T lymphocytes and the proportion of their subpopulations, but rather causes a significant decrease in the number of B lymphocytes and an increase in the number of NK cells. When comparing the DNA synthetic response to mitogenic and antigenic stimuli in patient's T and B lymphocytes using phytohaemagglutinin, pokeweed mitogen or allogenic mixed cells in separate in vitro cell cultures, the highest suppressive effect of thermal trauma is seen in the cells derived from the patients who had been given prophylactic IgG therapy. The presented data confirm that thermal trauma causes an immunosuppressive effect and indicate that prophylactic polyclonal IgG therapy increased the quantitative and functional suppression of the specific immune system while additionally increasing the cellular levels of the non-specific immune system, each system having been previously stimulated by thermal trauma.
在这项针对热烧伤患者的研究中，我们检测了标准治疗联合预防性多克隆免疫球蛋白G（IgG）治疗对免疫系统的体液和细胞成分、细胞表型及功能的影响，并将其与仅接受标准治疗的患者进行比较。定量外周血单核细胞分析显示，烧伤后3周，标准治疗患者的T淋巴细胞总数减少，自然杀伤（NK）细胞数量增加。我们发现，静脉注射IgG治疗对T淋巴细胞及其亚群比例没有重要影响，但会导致B淋巴细胞数量显著减少，NK细胞数量增加。在体外细胞培养中，分别使用植物血凝素、商陆有丝分裂原或同种异体混合细胞比较患者T和B淋巴细胞对有丝分裂原和抗原刺激的DNA合成反应时，在接受预防性IgG治疗的患者来源的细胞中，热烧伤的抑制作用最为明显。所呈现的数据证实热烧伤会导致免疫抑制作用，并表明预防性多克隆IgG治疗增强了特异性免疫系统的定量和功能抑制，同时还增加了非特异性免疫系统的细胞水平，此前每个系统均受到热烧伤的刺激。
