Isenschmid A, Marison I W, von Stockar U
Swiss Federal Institute of Technology, Lausanne.
J Biotechnol. 1995 May 1;39(3):229-37. doi: 10.1016/0168-1656(95)00018-l.
In order to study the potential use of supercritical CO2 extraction for the recovery of products from yeast cell cultures, the effects of CO2 on different yeast strains over a range of pressures and temperatures have been examined. Viability was shown to be dependent on temperature and dissolved CO2 concentration, and can be described by a sigmoidal (S-shaped) curve. Cell death was mainly due to an 'anaesthesia effect' rather than cell rupture. Important differences in sensitivity were observed for the strains studied, with the following order of resistance: Kluyveromyces fragilis > Saccharomyces cerevisiae > Candida utilis.
