Prombona A, Tabler M, Providaki M, Tsagris M
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Hellas, Heraklion, Greece.
Plant Mol Biol. 1995 Mar;27(6):1109-18. doi: 10.1007/BF00020884.
cDNA clones of a tomato protein, called Lycopersicum esculentum putative Mg(2+)-dependent ATPase (LeMA-1), were isolated from a cDNA library. Sequence comparison of the tomato protein with other genes in the database revealed that the protein is highly homologous to a human protein called TBP-1 and a yeast Tat-binding-analogue protein YTA1A. All three proteins belong to the recently discovered protein family of putative Mg(2+)-dependent ATPases and form within this family a subgroup of proteins involved in controlled protein degradation and possibly also in transcriptional regulation. Expression of the mRNA of LeMA-1 could be monitored in several plant tissues. LeMA-1 is the first member of this subgroup of proteins isolated from plants.
从一个cDNA文库中分离出了一种番茄蛋白的cDNA克隆，该蛋白被称为番茄（Lycopersicum esculentum）假定的Mg(2+)依赖性ATP酶（LeMA-1）。将该番茄蛋白与数据库中的其他基因进行序列比较后发现，该蛋白与一种名为TBP-1的人类蛋白以及一种酵母Tat结合类似蛋白YTA1A高度同源。这三种蛋白都属于最近发现的假定的Mg(2+)依赖性ATP酶蛋白家族，并且在这个家族中形成了一个与受控蛋白质降解以及可能还与转录调控有关的蛋白质亚组。可以在几种植物组织中监测LeMA-1的mRNA表达。LeMA-1是从植物中分离出的该蛋白质亚组的首个成员。
