Bretnall A E, Clarke G S
Bristol-Myers Squibb Pharmaceutical Research Institute, International Development Laboratories, Moreton, UK.
J Chromatogr A. 1995 May 12;700(1-2):173-8. doi: 10.1016/0021-9673(95)00112-z.
A micellar electrokinetic chromatography method was optimised for the separation of the six cardiovascular drugs atenolol, nicardipine, nifedipine, diltiazem, verapamil, and amlodipine by investigating the effects of pH, sodium dodecyl sulphate (SDS) concentration, selection and concentration of organic modifier. An electrophoresis buffer of 100 mM borate pH 8.1 containing 50 mM SDS and 15% (v/v) acetone was found to provide the optimum separation with respect to resolution and migration time.
通过研究pH值、十二烷基硫酸钠（SDS）浓度、有机改性剂的选择和浓度对分离效果的影响，优化了胶束电动色谱法以分离六种心血管药物，即阿替洛尔、尼卡地平、硝苯地平、地尔硫卓、维拉帕米和氨氯地平。结果发现，含有50 mM SDS和15%（v/v）丙酮的100 mM pH 8.1硼酸盐电泳缓冲液在分离度和迁移时间方面提供了最佳分离效果。
