Selleck M A, Bronner-Fraser M
Developmental Biology Center, University of California at Irvine 92717, USA.
Development. 1995 Feb;121(2):525-38. doi: 10.1242/dev.121.2.525.
We have investigated the lineage and tissue interactions that result in avian neural crest cell formation from the ectoderm. Presumptive neural plate was grafted adjacent to non-neural ectoderm in whole embryo culture to examine the role of tissue interactions in ontogeny of the neural crest. Our results show that juxtaposition of non-neural ectoderm and presumptive neural plate induces the formation of neural crest cells. Quail/chick recombinations demonstrate that both the prospective neural plate and the prospective epidermis can contribute to the neural crest. When similar neural plate/epidermal confrontations are performed in tissue culture to look at the formation of neural crest derivatives, juxtaposition of epidermis with either early (stages 4-5) or later (stages 6-10) neural plate results in the generation of both melanocytes and sympathoadrenal cells. Interestingly, neural plates isolated from early stages form no neural crest cells, whereas those isolated later give rise to melanocytes but not crest-derived sympathoadrenal cells. Single cell lineage analysis was performed to determine the time at which the neural crest lineage diverges from the epidermal lineage and to elucidate the timing of neural plate/epidermis interactions during normal development. Our results from stage 8 to 10+ embryos show that the neural plate/neural crest lineage segregates from the epidermis around the time of neural tube closure, suggesting that neural induction is still underway at open neural plate stages.
我们研究了外胚层产生鸟类神经嵴细胞的谱系和组织相互作用。在全胚胎培养中，将预定神经板移植到非神经外胚层附近，以研究组织相互作用在神经嵴个体发育中的作用。我们的结果表明，非神经外胚层与预定神经板并置可诱导神经嵴细胞的形成。鹌鹑/鸡重组实验表明，预期的神经板和预期的表皮都可以产生神经嵴。当在组织培养中进行类似的神经板/表皮对置实验以观察神经嵴衍生物的形成时，表皮与早期（4-5期）或晚期（6-10期）神经板并置会产生黑素细胞和交感肾上腺细胞。有趣的是，早期分离的神经板不形成神经嵴细胞，而后期分离的神经板则产生黑素细胞，但不产生源自神经嵴的交感肾上腺细胞。进行单细胞谱系分析以确定神经嵴谱系与表皮谱系分离的时间，并阐明正常发育过程中神经板/表皮相互作用的时间。我们对8至10+期胚胎的研究结果表明，神经板/神经嵴谱系在神经管闭合时左右与表皮分离，这表明在开放神经板阶段神经诱导仍在进行。
