Moury J D, Jacobson A G
Department of Zoology, University of Texas, Austin 78712-1064.
Dev Biol. 1990 Oct;141(2):243-53. doi: 10.1016/0012-1606(90)90380-2.
We address the question of whether neural crest cells originate from the neural plate, from the epidermis, or from both of these tissues. Our past studies revealed that a neural fold and neural crest cells could arise at any boundary created between epidermis and neural plate. To examine further the formation of neural crest cells at newly created boundaries in embryos of a urodele (Ambystoma mexicanum), we replace a portion of the neural folds of an albino host with either epidermis or neural plate from a normally pigmented donor. We then look for cells that contain pigment granules in the neural crest and its derivatives in intact and sectioned host embryos. By tracing cells in this manner, we find that cells from neural plate transplants give rise to melanocytes and (in one case) become part of a spinal ganglion, and we find that epidermal transplants contribute cells to the spinal and cranial ganglia. Thus neural crest cells arise from both the neural plate and the epidermis. These results also indicate that neural crest induction is (at least partially) governed by local reciprocal interactions between epidermis and neural plate at their common boundary.
我们探讨了神经嵴细胞是起源于神经板、表皮，还是这两种组织都有。我们过去的研究表明，神经褶和神经嵴细胞可以出现在表皮与神经板之间形成的任何边界处。为了进一步研究有尾目动物（墨西哥钝口螈）胚胎中新形成边界处神经嵴细胞的形成，我们用正常色素沉着供体的表皮或神经板替换白化病宿主的一部分神经褶。然后，我们在完整的和切片的宿主胚胎中寻找神经嵴及其衍生物中含有色素颗粒的细胞。通过这种方式追踪细胞，我们发现来自神经板移植的细胞产生黑色素细胞，并且（在一个案例中）成为脊髓神经节的一部分，并且我们发现表皮移植为脊髓和颅神经节贡献细胞。因此，神经嵴细胞起源于神经板和表皮。这些结果还表明，神经嵴诱导（至少部分）受表皮和神经板在其共同边界处的局部相互作用控制。
