来自细胞表面的转录调控：跨膜蛋白FecR的构象变化导致大肠杆菌中铁柠檬酸转运基因的转录改变。
Transcriptional regulation from the cell surface: conformational changes in the transmembrane protein FecR lead to altered transcription of the ferric citrate transport genes in Escherichia coli.
作者信息
Wriedt K, Angerer A, Braun V
机构信息
Universität Tübingen, Germany.
出版信息
J Bacteriol. 1995 Jun;177(11):3320-2. doi: 10.1128/jb.177.11.3320-3322.1995.
Abstract
Ferric citrate induces the ferric citrate transport system in Escherichia coli without being taken up into cells. The cytoplasmic transmembrane protein FecR, required for the response to ferric citrate, was found to be cleaved by a cellular protease. FecR protein produced by fecR mutants impaired or constitutive in fecA transcription was protease resistant, indicating that conformational changes affect proper functioning of FecR.
摘要
柠檬酸铁可诱导大肠杆菌中的柠檬酸铁转运系统，而自身不被细胞摄取。已发现，响应柠檬酸铁所必需的细胞质跨膜蛋白FecR会被一种细胞蛋白酶切割。由fecR突变体产生的、在fecA转录中受损或组成型表达的FecR蛋白具有蛋白酶抗性，这表明构象变化会影响FecR的正常功能。
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