大肠杆菌K-12热休克反应期间的拓扑异构酶活性
Topoisomerase activity during the heat shock response in Escherichia coli K-12.
作者信息
Camacho-Carranza R, Membrillo-Hernández J, Ramírez-Santos J, Castro-Dorantes J, Chagoya de Sánchez V, Gómez-Eichelmann M C
机构信息
Departamento de Biología Molecular, Universidad Nacional Autónoma de México, México, D.F.
出版信息
J Bacteriol. 1995 Jun;177(12):3619-22. doi: 10.1128/jb.177.12.3619-3622.1995.
Abstract
During the upshift of temperature from 30 to 42, 45, 47, or 50 degrees C, an increase in the level of supercoiling of a reporter plasmid was observed. This increase was present in groE and dnaK mutants but was inhibited in cells treated with chloramphenicol and novobiocin. The intracellular [ATP]/[ADP] ratio increased rapidly after an upshift in temperature from 30 to 47 degrees C and then decreased to reach a level above that observed at 30 degrees C. These results suggest that gyrase and proteins synthesized during heat shock are responsible for the changes seen in plasmid supercoiling. Proteins GroE and DnaK are probably not involved in this phenomenon.
摘要
在温度从30℃上升至42℃、45℃、47℃或50℃的过程中，观察到报告质粒的超螺旋水平增加。这种增加在groE和dnaK突变体中存在，但在用氯霉素和新生霉素处理的细胞中受到抑制。当温度从30℃上升至47℃后，细胞内[ATP]/[ADP]比值迅速增加，然后下降至高于30℃时观察到的水平。这些结果表明，热休克期间合成的gyrase和蛋白质是质粒超螺旋变化的原因。蛋白质GroE和DnaK可能不参与这一现象。
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本文引用的文献
1
Characterization of twenty-six new heat shock genes of Escherichia coli.大肠杆菌26个新热休克基因的特性分析
J Bacteriol. 1993 Aug;175(16):5242-52. doi: 10.1128/jb.175.16.5242-5252.1993.
2
Topoisomerase mutations affect the relative abundance of many Escherichia coli proteins.拓扑异构酶突变会影响许多大肠杆菌蛋白质的相对丰度。
Mol Microbiol. 1993 Nov;10(3):473-81. doi: 10.1111/j.1365-2958.1993.tb00920.x.
3
Effect of nalidixic acid and novobiocin on pBR322 genetic expression in Escherichia coli minicells.萘啶酸和新生霉素对大肠杆菌微小细胞中pBR322基因表达的影响。
J Bacteriol. 1981 Dec;148(3):745-52. doi: 10.1128/jb.148.3.745-752.1981.
4
Regulation of bacterial DNA supercoiling: plasmid linking numbers vary with growth temperature.细菌DNA超螺旋的调控：质粒连环数随生长温度而变化。
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4046-50. doi: 10.1073/pnas.81.13.4046.
5
Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling.大肠杆菌DNA促旋酶基因的调控：DNA超螺旋的稳态控制
Cell. 1983 Aug;34(1):105-13. doi: 10.1016/0092-8674(83)90140-x.
6
Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.大肠杆菌DNA拓扑异构酶I突变体：超螺旋增加可通过gyrase基因附近的突变得到纠正。
Cell. 1982 Nov;31(1):35-42. doi: 10.1016/0092-8674(82)90402-0.
7
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8
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9
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10
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J Bacteriol. 1987 Oct;169(10):4499-506. doi: 10.1128/jb.169.10.4499-4506.1987.
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