大肠杆菌野生型和rpoS稳定期细胞中的质粒DNA超螺旋和促旋酶活性。
Plasmid DNA supercoiling and gyrase activity in Escherichia coli wild-type and rpoS stationary-phase cells.
作者信息
Reyes-Domínguez Yazmid, Contreras-Ferrat Gabriel, Ramírez-Santos Jesús, Membrillo-Hernández Jorge, Gómez-Eichelmann M Carmen
机构信息
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Mexico, D.F., Mexico.
出版信息
J Bacteriol. 2003 Feb;185(3):1097-100. doi: 10.1128/JB.185.3.1097-1100.2003.
Abstract
Stationary-phase cells displayed a distribution of relaxed plasmids and had the ability to recover plasmid supercoiling as soon as nutrients became available. Preexisting gyrase molecules in these cells were responsible for this recovery. Stationary-phase rpoS cells showed a bimodal distribution of plasmids and failed to supercoil plasmids after the addition of nutrients, suggesting that rpoS plays a role in the regulation of plasmid topology during the stationary phase.
摘要
稳定期细胞呈现出松弛质粒的分布，并且一旦有营养物质可用，就有能力恢复质粒超螺旋结构。这些细胞中预先存在的促旋酶分子负责这种恢复。稳定期的rpoS细胞呈现出质粒的双峰分布，并且在添加营养物质后无法使质粒超螺旋化，这表明rpoS在稳定期质粒拓扑结构的调节中发挥作用。
相似文献
1
Plasmid DNA supercoiling and gyrase activity in Escherichia coli wild-type and rpoS stationary-phase cells.大肠杆菌野生型和rpoS稳定期细胞中的质粒DNA超螺旋和促旋酶活性。
J Bacteriol. 2003 Feb;185(3):1097-100. doi: 10.1128/JB.185.3.1097-1100.2003.
2
Role of DNA supercoiling and rpoS sigma factor in the osmotic and growth phase-dependent induction of the gene osmE of Escherichia coli K12.DNA超螺旋和rpoS σ因子在大肠杆菌K12的osmE基因的渗透压和生长阶段依赖性诱导中的作用。
J Mol Biol. 1997 Oct 17;273(1):75-83. doi: 10.1006/jmbi.1997.1308.
3
Rates of gyrase supercoiling and transcription elongation control supercoil density in a bacterial chromosome.拓扑异构酶超螺旋和转录延伸的速率控制细菌染色体的超螺旋密度。
PLoS Genet. 2012;8(8):e1002845. doi: 10.1371/journal.pgen.1002845. Epub 2012 Aug 16.
4
Sigma s regulates pLS1 maintenance in stationary-phase Escherichia coli.
FEMS Microbiol Lett. 1996 Jan 1;135(1):45-50. doi: 10.1111/j.1574-6968.1996.tb07964.x.
5
Topological insulators inhibit diffusion of transcription-induced positive supercoils in the chromosome of Escherichia coli.拓扑绝缘体抑制大肠杆菌染色体中转录诱导的正超螺旋扩散。
Mol Microbiol. 2005 Jan;55(2):601-10. doi: 10.1111/j.1365-2958.2004.04411.x.
6
Molecular basis of the supercoil deficit induced by the mini-F plasmid partition complex.微小F质粒分配复合体诱导的超螺旋缺陷的分子基础
J Biol Chem. 2009 Jan 2;284(1):165-173. doi: 10.1074/jbc.M802752200. Epub 2008 Nov 10.
7
RpoS regulates a novel type of plasmid DNA transfer in Escherichia coli.RpoS 调控大肠杆菌中一种新型的质粒 DNA 转移。
PLoS One. 2012;7(3):e33514. doi: 10.1371/journal.pone.0033514. Epub 2012 Mar 16.
8
Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes.测量细菌染色体中的体内超螺旋动力学和转录延伸率。
Methods Mol Biol. 2017;1624:17-27. doi: 10.1007/978-1-4939-7098-8_2.
9
The rpoS gene regulates OP2, an operon for the lower pathway of xylene catabolism on the TOL plasmid, and the stress response in Pseudomonas putida mt-2.rpoS基因调控OP2，OP2是TOL质粒上二甲苯分解代谢下游途径的一个操纵子，同时也调控恶臭假单胞菌mt-2中的应激反应。
Mol Gen Genet. 1998 Jul;259(1):72-8. doi: 10.1007/s004380050790.
10
Growth rate toxicity phenotypes and homeostatic supercoil control differentiate Escherichia coli from Salmonella enterica serovar Typhimurium.生长速率毒性表型和稳态超螺旋控制区分了大肠杆菌与鼠伤寒沙门氏菌。
J Bacteriol. 2007 Aug;189(16):5839-49. doi: 10.1128/JB.00083-07. Epub 2007 Mar 30.
引用本文的文献
1
Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria.单细胞微流控技术有助于快速定量革兰氏阴性菌中抗生素的积累。
Lab Chip. 2020 Aug 7;20(15):2765-2775. doi: 10.1039/d0lc00242a. Epub 2020 Jul 2.
2
Supercoil Levels in and Chromosomes Are Regulated by the C-Terminal 35⁻38 Amino Acids of GyrA.螺旋酶A亚基C末端35至38个氨基酸调控大肠杆菌和肺炎链球菌染色体中的超螺旋水平。
Microorganisms. 2019 Mar 15;7(3):81. doi: 10.3390/microorganisms7030081.
3
Vector Design for Improved DNA Vaccine Efficacy, Safety and Production.用于提高DNA疫苗效力、安全性和产量的载体设计
Vaccines (Basel). 2013 Jun 25;1(3):225-49. doi: 10.3390/vaccines1030225.
4
Role of chaperones and ATP synthase in DNA gyrase reactivation in Escherichia coli stationary-phase cells after nutrient addition.伴侣蛋白和ATP合酶在添加营养物后大肠杆菌稳定期细胞中DNA促旋酶再激活中的作用。
Springerplus. 2014 Nov 6;3:656. doi: 10.1186/2193-1801-3-656. eCollection 2014.
5
Micro-minicircle Gene Therapy: Implications of Size on Fermentation, Complexation, Shearing Resistance, and Expression.微迷你环基因治疗：大小对发酵、复合物形成、抗剪切和表达的影响。
Mol Ther Nucleic Acids. 2014 Jan 7;2(1):e140. doi: 10.1038/mtna.2013.67.
6
General stress response signalling: unwrapping transcription complexes by DNA relaxation via the sigma38 C-terminal domain.一般应激反应信号传导：通过σ38 C末端结构域使DNA松弛来解开转录复合物。
Mol Microbiol. 2008 Oct;70(2):369-78. doi: 10.1111/j.1365-2958.2008.06412.x. Epub 2008 Aug 22.
7
Growth rate toxicity phenotypes and homeostatic supercoil control differentiate Escherichia coli from Salmonella enterica serovar Typhimurium.生长速率毒性表型和稳态超螺旋控制区分了大肠杆菌与鼠伤寒沙门氏菌。
J Bacteriol. 2007 Aug;189(16):5839-49. doi: 10.1128/JB.00083-07. Epub 2007 Mar 30.
8
Long-term experimental evolution in Escherichia coli. XII. DNA topology as a key target of selection.大肠杆菌中的长期实验进化。十二、DNA拓扑结构作为选择的关键靶点。
Genetics. 2005 Feb;169(2):523-32. doi: 10.1534/genetics.104.035717. Epub 2004 Oct 16.
9
Plasmid DNA supercoiling and survival in long-term cultures of Escherichia coli: role of NaCl.质粒DNA超螺旋与大肠杆菌长期培养中的存活：氯化钠的作用
J Bacteriol. 2003 Sep;185(17):5324-7. doi: 10.1128/JB.185.17.5324-5327.2003.
本文引用的文献
1
Alcohol-induced delay of viability loss in stationary-phase cultures of Escherichia coli.酒精诱导大肠杆菌稳定期培养物中活力丧失的延迟
J Bacteriol. 2002 Jun;184(11):2898-905. doi: 10.1128/JB.184.11.2898-2905.2002.
2
GyrI: a counter-defensive strategy against proteinaceous inhibitors of DNA gyrase.GyrI：一种针对DNA回旋酶蛋白质抑制剂的反防御策略。
EMBO Rep. 2002 Mar;3(3):261-7. doi: 10.1093/embo-reports/kvf038. Epub 2002 Feb 15.
3
DNA topoisomerases: structure, function, and mechanism.DNA拓扑异构酶：结构、功能及作用机制
Annu Rev Biochem. 2001;70:369-413. doi: 10.1146/annurev.biochem.70.1.369.
4
Functional modulation of Escherichia coli RNA polymerase.大肠杆菌RNA聚合酶的功能调节
Annu Rev Microbiol. 2000;54:499-518. doi: 10.1146/annurev.micro.54.1.499.
5
Roles of topoisomerases in maintaining steady-state DNA supercoiling in Escherichia coli.拓扑异构酶在维持大肠杆菌稳态DNA超螺旋中的作用。
J Biol Chem. 2000 Mar 17;275(11):8103-13. doi: 10.1074/jbc.275.11.8103.
6
A DNA architectural protein couples cellular physiology and DNA topology in Escherichia coli.一种DNA结构蛋白将大肠杆菌中的细胞生理学与DNA拓扑结构联系起来。
Mol Microbiol. 1999 Dec;34(5):953-64. doi: 10.1046/j.1365-2958.1999.01656.x.
7
Identification of DNA gyrase inhibitor (GyrI) in Escherichia coli.大肠杆菌中DNA促旋酶抑制剂（GyrI）的鉴定。
J Biol Chem. 1998 Jan 23;273(4):1933-8. doi: 10.1074/jbc.273.4.1933.
8
DNA topoisomerases.DNA拓扑异构酶
Annu Rev Biochem. 1996;65:635-92. doi: 10.1146/annurev.bi.65.070196.003223.
9
sbmC, a stationary-phase induced SOS Escherichia coli gene, whose product protects cells from the DNA replication inhibitor microcin B17.sbmC是一个在稳定期诱导表达的大肠杆菌SOS基因，其产物可保护细胞免受DNA复制抑制剂微菌素B17的影响。
Mol Microbiol. 1995 Oct;18(2):301-11. doi: 10.1111/j.1365-2958.1995.mmi_18020301.x.
10
Cyclic AMP receptor protein positively controls gyrA transcription and alters DNA topology after nutritional upshift in Escherichia coli.环腺苷酸受体蛋白正向调控大肠杆菌中gyrA的转录，并在营养上调后改变DNA拓扑结构。
J Bacteriol. 1996 Jun;178(11):3331-4. doi: 10.1128/jb.178.11.3331-3334.1996.
Zotero 插件