Bélec L, Matta M, Payan C, Grésenguet G, Tévi-Bénissan C, Pillot J
Unité d'Immunologie Microbienne, Institut Pasteur, Paris, France.
J Infect Dis. 1995 Jun;171(6):1615-8. doi: 10.1093/infdis/171.6.1615.
The failure to detect human immunodeficiency virus (HIV) antigen in cervicovaginal secretions (CVS) from HIV-infected women could be due in part to an in situ formation of immune complexes involving HIV antigens and cervicovaginal anti-HIV antibodies. CVS from 30 HIV-1-infected heterosexual women were assayed for p24 antigen detection by ELISA before and after acid disruption of immune complexes. Before acid treatment, free p24 antigen was detected in only 1 sample of CVS, whereas after acid dissociation, 4 (13.5%) of 30 samples had detectable and neutralizable p24 antigen. Adsorbent capacities of the CVS for recombinant p24 antigen, evaluated in an in vitro model, depended on both titer and avidity of cervicovaginal antibodies to HIV. In conclusion, local antibodies to HIV are able in vivo to bind HIV antigen within CVS and to participate in the immune exclusion of free virus particles or proteins.
未能在HIV感染女性的宫颈阴道分泌物（CVS）中检测到人类免疫缺陷病毒（HIV）抗原，部分原因可能是涉及HIV抗原和宫颈阴道抗HIV抗体的免疫复合物在原位形成。对30名感染HIV-1的异性恋女性的CVS进行检测，在免疫复合物酸破坏前后通过ELISA检测p24抗原。酸处理前，仅在1份CVS样本中检测到游离p24抗原，而酸解离后，30份样本中有4份（13.5%）具有可检测和可中和的p24抗原。在体外模型中评估的CVS对重组p24抗原的吸附能力，取决于宫颈阴道抗HIV抗体的滴度和亲和力。总之，HIV的局部抗体在体内能够结合CVS中的HIV抗原，并参与游离病毒颗粒或蛋白质的免疫排斥。
