Yang W, Summers J
Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131, USA.
J Virol. 1995 Jul;69(7):4029-36. doi: 10.1128/JVI.69.7.4029-4036.1995.
Linear hepadnavirus DNA in primary hepatocyte cultures efficiently participates in intra- and intermolecular nonhomologous recombination at its ends. The products of this recombination are (i) monomeric covalently closed circular DNAs (cccDNAs) with deletions and insertions around the site of joining and (ii) oligomeric forms in which monomers are joined near the ends in random orientation. A fraction of monomeric cccDNAs can serve as intermediates in further DNA replication through at least five generations of nonhomologous recombination in a process we call illegitimate replication. We suggest that the monomeric and oligomeric linear DNAs produced by illegitimate replication may be precursors of the integrated and other high-molecular-weight hepadnaviral DNA forms seen in chronic infection.
原代肝细胞培养物中的线性嗜肝DNA病毒DNA在其末端高效参与分子内和分子间非同源重组。这种重组的产物为：（i）在连接位点周围有缺失和插入的单体共价闭合环状DNA（cccDNA），以及（ii）寡聚体形式，其中单体在末端附近以随机方向连接。一部分单体cccDNA可作为进一步DNA复制的中间体，通过至少五代非同源重组，此过程我们称为非法复制。我们认为，非法复制产生的单体和寡聚线性DNA可能是慢性感染中所见整合型及其他高分子量嗜肝DNA病毒DNA形式的前体。
