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从慢性感染的土拨鼠肝细胞核中分离出的土拨鼠肝炎病毒DNA的新形式。

Novel forms of woodchuck hepatitis virus DNA isolated from chronically infected woodchuck liver nuclei.

作者信息

Rogler C E, Summers J

出版信息

J Virol. 1982 Dec;44(3):852-63. doi: 10.1128/JVI.44.3.852-863.1982.

DOI:10.1128/JVI.44.3.852-863.1982
PMID:6294334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256342/
Abstract

We cloned several unique forms of woodchuck hepatitis virus, a DNA virus closely related to hepatitis B virus, from a chronically infected woodchuck liver. Each of the three clones contained more than two genome equivalents of viral sequences with extensive rearrangements and no detectable cellular sequences. From the frequency by which they were isolated from a library of recombinant clones, we estimate that they are present in approximately one copy per cell. Of a total of 11 sites at which rearrangements were mapped in the clones, 10 occurred between segments of opposite polarity, and 1 occurred between segments of the same polarity. The possible significance of these findings to the persistence of virus production in infected cells is discussed.

摘要

我们从一只慢性感染的土拨鼠肝脏中克隆出了几种独特形式的土拨鼠肝炎病毒，这是一种与乙型肝炎病毒密切相关的DNA病毒。三个克隆中的每一个都包含超过两个基因组当量的病毒序列，这些序列有广泛的重排且未检测到细胞序列。根据从重组克隆文库中分离出它们的频率，我们估计它们在每个细胞中大约以一个拷贝的形式存在。在克隆中定位到重排的总共11个位点中，有10个发生在相反极性的片段之间，1个发生在相同极性的片段之间。本文讨论了这些发现对受感染细胞中病毒持续产生的可能意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/75262e39e977/jvirol00153-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/5b62cafc3edd/jvirol00153-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/b049729d991b/jvirol00153-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/f631a3b7f539/jvirol00153-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/75262e39e977/jvirol00153-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/5b62cafc3edd/jvirol00153-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/b049729d991b/jvirol00153-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/f631a3b7f539/jvirol00153-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb2/256342/75262e39e977/jvirol00153-0101-a.jpg

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1
Novel forms of woodchuck hepatitis virus DNA isolated from chronically infected woodchuck liver nuclei.从慢性感染的土拨鼠肝细胞核中分离出的土拨鼠肝炎病毒DNA的新形式。
J Virol. 1982 Dec;44(3):852-63. doi: 10.1128/JVI.44.3.852-863.1982.
2
Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from a chronically infected liver.来自慢性感染肝脏的整合土拨鼠肝炎病毒序列的克隆与结构分析
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Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from hepatocellular carcinomas of woodchucks.土拨鼠肝细胞癌中整合的土拨鼠肝炎病毒序列的克隆与结构分析
Cell. 1982 Jun;29(2):385-94. doi: 10.1016/0092-8674(82)90155-6.
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本文引用的文献

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Nucleotide sequence of a cloned woodchuck hepatitis virus genome: comparison with the hepatitis B virus sequence.克隆的土拨鼠肝炎病毒基因组的核苷酸序列：与乙肝病毒序列的比较。
J Virol. 1982 Jan;41(1):51-65. doi: 10.1128/JVI.41.1.51-65.1982.
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Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from hepatocellular carcinomas of woodchucks.土拨鼠肝细胞癌中整合的土拨鼠肝炎病毒序列的克隆与结构分析
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Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from a chronically infected liver.来自慢性感染肝脏的整合土拨鼠肝炎病毒序列的克隆与结构分析
J Virol. 1984 Jun;50(3):832-7. doi: 10.1128/JVI.50.3.832-837.1984.
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Isolation, characterization, and comparison of recombinant DNAs derived from genomes of human hepatitis B virus and woodchuck hepatitis virus.源自人乙型肝炎病毒和土拨鼠肝炎病毒基因组的重组DNA的分离、特性鉴定及比较。
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Replication of the genome of a hepatitis B--like virus by reverse transcription of an RNA intermediate.通过RNA中间体的逆转录来复制乙型肝炎样病毒的基因组。
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