在碱性条件下，采用基于氧化铝的高效液相色谱填充材料进行梯度洗脱对碱性药物进行系统毒理学分析。
Systematic toxicological analysis of basic drugs by gradient elution of an alumina-based HPLC packing material under alkaline conditions.
作者信息
Lambert W E, Meyer E, De Leenheer A P
机构信息
Laboratorium voor Toxicologie, Universiteit Gent, Belgium.
出版信息
J Anal Toxicol. 1995 Mar-Apr;19(2):73-8. doi: 10.1093/jat/19.2.73.
A high-performance liquid chromatographic system based on gradient elution of an alumina packing material coated with polybutadiene is presented. The chromatographic eluent is a mixture of methanol and water, both containing 0.0125M NaOH. The effluent is monitored by photodiode array detection. The system allows detection and identification of more than 130 toxicologically relevant substances (mainly basic compounds) and has already been successfully applied to screen more than 500 extracts of fresh or postmortem specimens (blood, urine, tissue homogenates, and stomach contents).
介绍了一种基于涂覆聚丁二烯的氧化铝填充材料梯度洗脱的高效液相色谱系统。色谱洗脱液是甲醇和水的混合物，二者均含有0.0125M的氢氧化钠。流出物通过光电二极管阵列检测进行监测。该系统能够检测和鉴定130多种具有毒理学相关性的物质（主要是碱性化合物），并且已经成功应用于筛查500多个新鲜或死后标本（血液、尿液、组织匀浆和胃内容物）的提取物。
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