Yeo S W, Gottschlich S, Harris J P, Keithley E M
Division of Otolaryngology-Head & Neck Surgery, University of California, San Diego, School of Medicine, La Jolla 92093-0666, USA.
Laryngoscope. 1995 Jun;105(6):623-8. doi: 10.1288/00005537-199506000-00012.
The diffusion pattern of horseradish peroxidase (HRP) injected into the scala tympani of the cochlear basal turn of guinea pigs was studied to test whether antigen presented in this manner can gain access to the endolymphatic sac. By two hours, HRP reaction product was found throughout the cochlea, with the greatest amounts in the spiral ligament, spiral limbus, basilar membrane, and organ of Corti. In several cochleas, very weak labeling was seen in the stria vascularis. HRP reaction product was maximal in the basal turn. By two hours, HRP reaction product was also observed in the endolymphatic sac lumen, epithelial cells, subepithelial tissue, and perisaccular connective tissue. It was more common in the proximal portion. At this time, macrophages within the lumen already appeared to have phagocytosed the HRP. By 72 hours after injection, the inner ear was cleared of HRP. The results of this study support the hypothesis that antigen in the scala tympani gains access to the endolymphatic sac lumen, where it may be presented by macrophages to the systemic immune system. Antigen most likely does not gain access to the endolymphatic space in the cochlea, but it gets to the endolymphatic sac through the perilymph and the perisaccular tissue.
将辣根过氧化物酶（HRP）注入豚鼠耳蜗基底转鼓阶，研究其扩散模式，以测试以这种方式呈现的抗原是否能够进入内淋巴囊。两小时后，在整个耳蜗中发现了HRP反应产物，在螺旋韧带、螺旋缘、基底膜和柯蒂氏器中含量最多。在几个耳蜗中，血管纹中可见非常微弱的标记。HRP反应产物在基底转中最多。两小时后，在内淋巴囊腔、上皮细胞、上皮下组织和囊周结缔组织中也观察到了HRP反应产物。在近端部分更常见。此时，腔内的巨噬细胞似乎已经吞噬了HRP。注射后72小时，内耳中的HRP被清除。本研究结果支持以下假设：鼓阶中的抗原进入内淋巴囊腔，在那里它可能由巨噬细胞呈递给全身免疫系统。抗原很可能无法进入耳蜗内淋巴间隙，但它通过外淋巴和囊周组织到达内淋巴囊。
