链球菌M蛋白抗体的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for streptococcal M protein antibodies.
作者信息
Russell H, Facklam R R, Edwards L R
出版信息
J Clin Microbiol. 1976 May;3(5):501-5. doi: 10.1128/jcm.3.5.501-505.1976.
The enzyme-linked immunosorbent assay (ELISA) described by Engvall and Perlmann, which uses antigen-coated tubes and enzyme-labeled anti-immunoglobulin, has been used for the detection of antibodies against streptococcal M protein. The antigen used in the assay was obtained by guanidine extraction of type M-12 streptococcal cell walls followed by hydroxyapatite chromatography. This antigen has the capacity to elicit bactericidal antibodies in rabbits. The results show that the ELISA is specific and highly sensitive for the detection of antibodies in rabbit and human antisera. Preliminary results suggest that, when M-12 antigen is used, the antibodies detected by ELISA are the same antibodies detected in the bactericidal test. The assay has been performed with human and rabbit sera. There was a 96% agreement between bactericidal and ELISA results with rabbit sera and 97.5% agreement with human sera. All bactericidal antibody-positive sera tested thus far yielded positive ELISA results.
英瓦尔和佩尔曼描述的酶联免疫吸附测定法（ELISA），该方法使用包被抗原的试管和酶标记的抗免疫球蛋白，已用于检测抗链球菌M蛋白的抗体。该测定法中使用的抗原是通过用胍提取M-12型链球菌细胞壁，然后进行羟基磷灰石层析获得的。这种抗原能够在兔体内引发杀菌抗体。结果表明，ELISA对于检测兔和人抗血清中的抗体具有特异性且高度灵敏。初步结果表明，当使用M-12抗原时，ELISA检测到的抗体与杀菌试验中检测到的抗体相同。该测定法已用于人和兔血清。兔血清的杀菌试验结果与ELISA结果的一致性为96%，人血清的一致性为97.5%。迄今为止，所有检测的杀菌抗体阳性血清的ELISA结果均为阳性。
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