通过酶联免疫吸附测定（ELISA）对肺炎支原体感染进行血清学诊断。
Serodiagnosis of M. pneumoniae infections by enzyme-linked immunosorbent assay (ELISA).
作者信息
Busolo F, Meloni G A
出版信息
Yale J Biol Med. 1983 Sep-Dec;56(5-6):517-21.
The enzyme-linked immunosorbent assay (ELISA) has been used for detection of antibodies against Mycoplasma pneumoniae. The results of ELISA and its sensitivity compared with other serological methods, such as complement fixation (CF), metabolic inhibition (MI), mycoplasmacidal test (MC), and radioimmunoprecipitation (RIP) are reported. ELISA and MC showed greater sensitivity than CF and MI, while RIP showed serum titer two- to 16-fold higher. ELISA was specific as determined using other human mycoplasma. A simplified method based on the determination of ELISA antibody end-point titer by a single serum dilution has been proposed. ELISA presented several advantages: sensitivity, rapidity, and low cost and, if adequately standardized, could become a reliable method for the serodiagnosis of M. pneumoniae infection.
酶联免疫吸附测定（ELISA）已用于检测抗肺炎支原体抗体。本文报道了ELISA的结果及其与其他血清学方法（如补体结合试验（CF）、代谢抑制试验（MI）、支原体杀菌试验（MC）和放射免疫沉淀试验（RIP））相比的灵敏度。ELISA和MC的灵敏度高于CF和MI，而RIP显示血清滴度高2至16倍。使用其他人类支原体检测表明ELISA具有特异性。本文提出了一种基于通过单次血清稀释测定ELISA抗体终点滴度的简化方法。ELISA具有几个优点：灵敏度高、速度快且成本低，如果进行充分标准化，可成为肺炎支原体感染血清学诊断的可靠方法。
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