用于检测肺炎支原体抗体的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for detection of Mycoplasma pneumoniae antibodies.
作者信息
Busolo F, Tonin E, Conventi L
出版信息
J Clin Microbiol. 1980 Jul;12(1):69-73. doi: 10.1128/jcm.12.1.69-73.1980.
Abstract
The enzyme-linked immunosorbent assay (ELISA) described by Engvall and Perlmann has been used for the detection of antibodies against Mycoplasma pneumoniae. These organisms, grown in flat-bottom wells of microtiter plates, were used as antigen. Preliminary results suggest that ELISA is specific and that its sensitivity is somewhere in between those of the metabolic inhibition and radioimmunoprecipitation methods. Unlike other serological methods, such as metabolic inhibition and mycoplasmacidal tests, in which the presence of antibiotics in sera may give false results, ELISA provided results that were not influenced by antibiotics. Furthermore, ELISA offers the possiblity of measuring not only immunoglobulin M, but also complement-independent antibodies, especially those of the immunoglobulin A class. Theoretical aspects concerning the different sensitivities of some serological reactions are discussed.
摘要
Engvall和Perlmann所描述的酶联免疫吸附测定法（ELISA）已用于检测抗肺炎支原体的抗体。这些在微量滴定板平底孔中培养的生物体被用作抗原。初步结果表明ELISA具有特异性，其灵敏度介于代谢抑制法和放射免疫沉淀法之间。与其他血清学方法不同，如代谢抑制法和支原体杀菌试验，血清中抗生素的存在可能会给出错误结果，而ELISA得出的结果不受抗生素影响。此外，ELISA不仅提供了测量免疫球蛋白M的可能性，还提供了测量非补体依赖性抗体的可能性，尤其是免疫球蛋白A类抗体。文中讨论了有关某些血清学反应不同灵敏度的理论方面。
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