Tato P, Castro A M, Rodríguez D, Soto R, Arechavaleta F, Molinari J L
Departamento de Microbiología e Inmunología, Universidad Nacional Autonóma de México, México, D.F.
Parasitol Res. 1995;81(3):181-7. doi: 10.1007/BF00937107.
A substance from Taenia solium metacestodes that decreases lymphocyte proliferation induced by concanavalin A was isolated. The molecular weight of this substance was estimated to be slightly more than 1,450 Da. Crude metacestode factor was fractionated through a Bio-gel P-6 column. Peak 1 showed suppressive activity. After incubation with RNase the substance lost its activity. Incubation of this material with trypsin or papain increased its suppressive activity. It was stable at boiling temperature for 10 min. The incubation of this substance with murine macrophages had no effect on [3H]-thymidine uptake by cocultured fresh splenic lymphocytes stimulated with concanavalin A. Conversely, cocultures of lymphocytes pretreated with the substance and fresh splenic lymphocytes showed a decreased incorporation of [3H]-thymidine. These results suggest that this substance is a RNA-peptide molecule whose RNA moiety accounts for its suppressive activity. The findings also suggest that in vivo the factor may be a modulator of the immune response.
从猪带绦虫中分离出一种可降低伴刀豆球蛋白A诱导的淋巴细胞增殖的物质。该物质的分子量估计略大于1450道尔顿。粗制的囊尾蚴因子通过Bio-gel P-6柱进行分级分离。峰1显示出抑制活性。与核糖核酸酶孵育后，该物质失去活性。用胰蛋白酶或木瓜蛋白酶孵育该物质可增强其抑制活性。它在沸腾温度下10分钟内稳定。该物质与小鼠巨噬细胞孵育对伴刀豆球蛋白A刺激的共培养新鲜脾淋巴细胞摄取[3H] - 胸腺嘧啶核苷没有影响。相反，用该物质预处理的淋巴细胞与新鲜脾淋巴细胞的共培养显示[3H] - 胸腺嘧啶核苷的掺入减少。这些结果表明该物质是一种RNA - 肽分子，其RNA部分决定了其抑制活性。研究结果还表明，在体内该因子可能是免疫反应的调节剂。
