评估鸡模型中隐孢子虫卵囊排出的定量方法。

Quantitative method to assess Cryptosporidium oocyst shedding in the chicken model.

作者信息

Varga I, Sréter T, Békési L

机构信息

Department of Parasitology and Zoology, University of Veterinary Science, Budapest, Hungary.

出版信息

Parasitol Res. 1995;81(3):262-4. doi: 10.1007/BF00937120.

DOI:10.1007/BF00937120

PMID:7770434

Abstract

An unsophisticated and fairly sensitive quantitative method and its grounds are described, which proved useful to assess Cryptosporidium oocyst shedding in the chicken model. The method is based on the rather slow sedimentation of oocysts. A threshold of sensitivity ranging between 5,000 and 10,000 oocysts/g feces was established for this technique. There was good agreement between triplicate assays over a wide range of oocyst concentrations.

摘要

描述了一种简单且相当灵敏的定量方法及其依据，该方法被证明在评估鸡模型中隐孢子虫卵囊排出情况时很有用。该方法基于卵囊沉降相对缓慢的特性。为该技术确定了每克粪便中5000至10000个卵囊的灵敏度阈值。在广泛的卵囊浓度范围内，三次重复测定之间具有良好的一致性。