Skerrett H E, Holland C V
Department of Zoology, Trinity College, 2, Dublin, Ireland.
Vet Parasitol. 2001 Jan 20;94(4):239-46. doi: 10.1016/s0304-4017(00)00405-2.
Levels of Cryptosporidium infection in a group of red deer were monitored over a period of 1 year. Faecal samples were examined on an approximate monthly basis from adult hinds and calves for the presence of Cryptosporidium oocysts. The water-ether sedimentation method followed by sucrose flotation and a monoclonal antibody identification procedure were used. It was found that apparently healthy adult deer were shedding low numbers of oocysts in their faeces throughout the year and that there appeared to be a periparturient increase in the numbers of oocysts shed. Samples taken from 6-month-old deer calves, both in-house and on pasture, had low numbers of Cryptosporidium oocysts, indicating that the calves were also asymptomatically shedding oocysts.
在一年的时间里对一群马鹿的隐孢子虫感染水平进行了监测。大约每月对成年母鹿和幼鹿的粪便样本进行检查，以检测隐孢子虫卵囊的存在。采用水-乙醚沉淀法，随后进行蔗糖浮选和单克隆抗体鉴定程序。结果发现，看似健康的成年鹿全年粪便中排出的卵囊数量较少，且在围产期排出的卵囊数量似乎有所增加。从圈养和牧场中6个月大的鹿犊采集的样本中，隐孢子虫卵囊数量较少，这表明这些鹿犊也在无症状地排出卵囊。
