8例慢性心功能不全患者血清中游离地高辛的测定。
Determination of free digoxin in sera of 8 patients with chronic cardiac insufficiency.
作者信息
Li J H, Pan H, Wu Z P, Chen Y L, Chu X M, Lin S S
机构信息
Department of Pharmacology, General Hospital of Nanjing Command, PLA, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 Jan;16(1):47-50.
AIM
To establish a method for the determination of free digoxin in serum for clinical use and to study the relationship between the free and total digoxin concentrations in chronic cardiac insufficiency patients receiving digoxin with different renal function.
METHODS
The ultrafiltration with fluorescence polarization immunoassay was used to determine the concentration of free digoxin.
RESULTS
The concentrations of digoxin standards in serum were 0.96, 1.92, and 3.84 nmol.L-1. The relative standard deviation was < 7% for intra-day and < 6% for inter-day determinations. The average recovery was 99.95 +/- 2.18%. The ratio of free/total digoxin in chronic cardiac insufficiency patients with renal dysfunction was lower than that in patients with normal kidneys (63.5 +/- 4.7% vs 75.1 +/- 3.9%, P < 0.01).
CONCLUSION
The present method is simple and reliable. In these patients there is an over-measurement for total digoxin concentration, suggesting the presence of elevated endogenous digoxin-like immunoreactive substances.
目的
建立一种临床可用的血清中游离地高辛的测定方法，并研究不同肾功能的慢性心功能不全患者使用地高辛时游离地高辛浓度与总地高辛浓度之间的关系。
方法
采用荧光偏振免疫法超滤测定游离地高辛浓度。
结果
血清中地高辛标准品浓度分别为0.96、1.92和3.84 nmol·L-1。日内相对标准偏差<7%，日间测定相对标准偏差<6%。平均回收率为99.95±2.18%。肾功能不全的慢性心功能不全患者游离/总地高辛比值低于肾功能正常患者（63.5±4.7%对75.1±3.9%，P<0.01）。
结论
本方法简便可靠。在这些患者中，总地高辛浓度存在测量过度的情况，提示内源性地高辛样免疫反应性物质升高。
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