Nobelmann B, Lengeler J W
Universität Osnabrück, Fachbereich Biologie/Chemie, Germany.
Biochim Biophys Acta. 1995 May 17;1262(1):69-72. doi: 10.1016/0167-4781(95)00053-j.
The sequence of the gat operon for galactitol (Gat) utilization from a wild-type isolate of Escherichia coli, strain EC3132, is presented. The operon comprises 7 open reading frames (ORFs) called gatYZABCDR. The genes are transcribed from a promoter located upstream of gatY. Genes gatABC encode the substrate-specific domains IIA, IIB and IIC of a galactitol-specific Enzyme II (EIIGat) of the phospho enol pyruvate-dependent carbohydrate:phosphotransferase system (PTS); gatD encodes an NAD-dependent Gat 1-phosphate dehydrogenase; and gatY an enzyme which hydrolyses tagatose 1,6-bisphosphate; gene gatZ is required in a cell to show a Gat+ phenotype, but its physiological function has not yet been identified; gatR encodes a repressor for the gat operon. All genes are highly similar to the gat genes from E. coli K-12; in this organism they map at 46.70 min of the gene map, equivalent to about 2180-2186 kbp.
本文展示了来自大肠杆菌野生型菌株EC3132的用于利用半乳糖醇（Gat）的gat操纵子序列。该操纵子由7个开放阅读框（ORF）组成，分别称为gatYZABCDR。这些基因从位于gatY上游的启动子转录而来。基因gatABC编码磷酸烯醇丙酮酸依赖性碳水化合物：磷酸转移酶系统（PTS）中半乳糖醇特异性酶II（EIIGat）的底物特异性结构域IIA、IIB和IIC；gatD编码一种NAD依赖性的Gat 1-磷酸脱氢酶；gatY编码一种水解塔格糖1,6-二磷酸的酶；细胞中需要基因gatZ才能表现出Gat+表型，但其生理功能尚未确定；gatR编码gat操纵子的阻遏物。所有基因都与大肠杆菌K-12的gat基因高度相似；在该生物体中，它们位于基因图谱的46.70分钟处，相当于约2180 - 2186 kbp。
