Seong C, Jeong S, Park D, Yoon J, Oh Y, Yim J, Han K, Baek K
Department and Institute of Genetic Engineering and Natural Sciences, Department of Genetic Engineering, Kyung Hee University, Yongin City 449-701, South Korea.
Biochim Biophys Acta. 1998 Oct 14;1388(1):273-8. doi: 10.1016/s0167-4838(98)00203-9.
We have isolated and characterized the cDNA and the genomic DNA encoding Drosophila melanogaster pterin 4alpha-carbinolamine dehydratase (PCD). The amino acid sequence deduced from the cDNA sequence was very similar to those of PCDs previously reported in other species (19-57% identity). The protein coding region of the cDNA was expressed in E. coli as a histidine fusion protein, and the expressed protein proved to have PCD activity. The characterization of the Drosophila genomic clone revealed that the Drosophila PCD gene is interrupted by two introns. The potential promoter region, deduced from the determination of the transcription start point (tsp), lacks the distinct TATAAA box consensus sequence.
