Wening J V, Marquardt H, Katzer A, Jungbluth K H, Marquardt H
Department of Trauma and Reconstructive Surgery, University of Hamburg, Medical School, Germany.
Biomaterials. 1995 Mar;16(4):337-40. doi: 10.1016/0142-9612(95)93262-c.
Toxicity and mutagenicity of Kevlar 49 (PPPT; poly-para-phenylene-terephthalamide) was tested in six strains of Salmonella typhimurium (Ames test; TA97, TA98, TA100, TA102, TA1535, TA1537) with and without an external metabolic activation system (S9), as well as in a mammalian cell mutagenesis assay using V79 Chinese hamster cells. For the Ames test, liquid preincubation, which is considered particularly sensitive, was used. The cells were incubated for 24 h at a temperature of 37 degrees C either directly with Kevlar49 or with ethanol- or chloroform-extracted Kevlar49. The experiments were performed at least twice. The Ames test with six different Salmonella typhimurium strains featuring either base pair substitution or frameshift mutations revealed no cytotoxic or mutagenic activity of Kevlar49. In the mammalian cell mutagenesis assay, using 8-azaguanine (AG) as a selective agent, Kevlar49 was also devoid of cytotoxic or mutagenic activity. Both tests have to be regarded as an initial exploratory screening due to the chosen testing conditions and should be supplemented by tests at different temperatures.
在有和没有外部代谢激活系统（S9）的情况下，在六种鼠伤寒沙门氏菌菌株（艾姆斯试验；TA97、TA98、TA100、TA102、TA1535、TA1537）中测试了凯夫拉尔49（PPPT；聚对苯二甲酰对苯二胺）的毒性和诱变性，以及在使用V79中国仓鼠细胞的哺乳动物细胞诱变试验中进行了测试。对于艾姆斯试验，使用了被认为特别敏感的液体预孵育法。细胞在37摄氏度的温度下直接与凯夫拉尔49或与乙醇或氯仿提取的凯夫拉尔49孵育24小时。实验至少进行了两次。对六种不同的具有碱基对替换或移码突变的鼠伤寒沙门氏菌菌株进行的艾姆斯试验显示，凯夫拉尔49没有细胞毒性或诱变活性。在哺乳动物细胞诱变试验中，使用8-氮杂鸟嘌呤（AG）作为选择剂，凯夫拉尔49也没有细胞毒性或诱变活性。由于所选择的测试条件，这两种测试都应被视为初步探索性筛选，并且应该通过在不同温度下的测试进行补充。
