Marin-Garcia J, Goldenthal M J, Ananthakrishnan R, Mirvis D
Molecular Cardiology Institute, Highland Park, NJ 08904, USA.
Biochem Mol Biol Int. 1995 Mar;35(3):651-9.
Effects of myocardial ischemia on mitochondrial enzymes and mitochondrial DNA (mtDNA) were examined using the model of Ameroid constriction of canine cardiac vessels. Endocardium supplied by constricted coronary arteries was found to have significantly lower citrate synthase and complex IV activities compared to values obtained from either epicardium supplied by constricted vessels or endocardium supplied by unconstricted coronary arteries. Neither significant differences in mtDNA copy number nor changes in respiratory complexes I, III and V were detected. These results suggest that highly localized, specific mitochondrial enzyme changes result from chronic myocardial ischemia.
利用犬类心脏血管阿梅罗氏缩窄模型，研究了心肌缺血对线粒体酶和线粒体DNA（mtDNA）的影响。结果发现，与狭窄血管供应的心外膜或未狭窄冠状动脉供应的心内膜相比，狭窄冠状动脉供应的心内膜柠檬酸合酶和复合物IV活性显著降低。未检测到mtDNA拷贝数的显著差异，也未检测到呼吸复合物I、III和V的变化。这些结果表明，慢性心肌缺血会导致高度局部化的特异性线粒体酶变化。
