Marin-Garcia J, Goldenthal M J, Ananthakrishnan R, Mirvis D
Molecular Cardiology Institute, Highland Park, NJ 08904, USA.
Biochem Mol Biol Int. 1996 Nov;40(5):1057-65. doi: 10.1080/15216549600201693.
The effect of myocardial ischemia on mitochondrial DNA (mtDNA) structure and the presence of specific mtDNA deletions was determined using the model of Ameroid constriction of canine coronary arteries. The incidence of specific deletions was high in both the endocardial and epicardial tissues perfused by the occluded vessel as compared to myocardial tissues perfused by the unconstricted vessels. Our results show that specific mtDNA deletions similar to the 5 kb and 7.4 kb human mtDNA deletions occur following canine myocardial ischemia. However the presence of these deletions did not correlate with specific mitochondrial enzyme defects.
利用犬冠状动脉阿梅氏环缩窄模型，确定了心肌缺血对线粒体DNA（mtDNA）结构的影响以及特定mtDNA缺失的存在情况。与未受缩窄血管灌注的心肌组织相比，受阻塞血管灌注的心内膜和心外膜组织中特定缺失的发生率较高。我们的结果表明，犬心肌缺血后会出现与人类mtDNA 5 kb和7.4 kb缺失类似的特定mtDNA缺失。然而，这些缺失的存在与特定的线粒体酶缺陷并无关联。
