Magoulas A, Sophronides K, Patarnello T, Hatzilaris E, Zouros E
Institute of Marine Biology of Crete, Greece.
Mol Mar Biol Biotechnol. 1995 Jun;4(2):110-6.
We present a restriction site map for the mitochondrial DNA of the gilthead sea bream and the consensus sequence for a region of the mitochondrial cytochrome b gene. Restriction site and nucleotide sequence analysis was used to obtain individual mitotype profiles in a broodstock used for genetic studies. Eight mitotypes were detected among 33 animals assayed. In combination with hypervariable nuclear markers, this variation can be used to identify the parents of any offspring in mass matings and, also, to determine which of the two parents donated the egg and who donated the sperm. On the basis of restriction site variation, the Greek stock analyzed here is very similar to an Israeli stock described in an earlier publication. It is not known if this similarity will hold when the Israeli stock is examined for sequence variation.
我们展示了金头鲷线粒体DNA的限制性酶切图谱以及线粒体细胞色素b基因一个区域的共有序列。利用限制性酶切位点和核苷酸序列分析，在用于遗传研究的亲鱼群体中获得了个体的线粒体基因型谱。在检测的33只动物中检测到了8种线粒体基因型。结合高变核标记，这种变异可用于确定大规模交配中任何后代的亲本，还能确定两个亲本中哪一个提供了卵子，哪一个提供了精子。基于限制性酶切位点变异，此处分析的希腊种群与早期出版物中描述的以色列种群非常相似。尚不清楚对以色列种群进行序列变异检测时这种相似性是否依然存在。
